Tipepidine activates VTA dopamine neuron via inhibiting dopamine D2 receptor-mediated inward rectifying K+ current

• Published in: Neuroscience Vol. 252; pp. 24 - 34
• Main Authors: Hamasaki, R, Shirasaki, T, Soeda, F, Takahama, K
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 12.11.2013; Elsevier
• Subjects: acutely dissociated neuron; Animals; Antidepressive Agents - pharmacology; Biological and medical sciences; dopamine; Dopaminergic Neurons - drug effects; Dopaminergic Neurons - metabolism; Fundamental and applied biological sciences. Psychology; G protein-coupled inwardly rectifying potassium channel; G Protein-Coupled Inwardly-Rectifying Potassium Channels - drug effects; G Protein-Coupled Inwardly-Rectifying Potassium Channels - metabolism; Immunohistochemistry; Neurology; patch clamp; Patch-Clamp Techniques; Piperidines - pharmacology; Rats; Rats, Wistar; Receptors, Dopamine D2 - drug effects; Receptors, Dopamine D2 - metabolism; tipepidine; ventral tegmental area; Ventral Tegmental Area - drug effects; Ventral Tegmental Area - metabolism; Vertebrates: nervous system and sense organs; NAT; baclofen-induced current; VTA; acutely dissociated neuron; G-protein-coupled receptor; phosphate buffer; obsessive–compulsive disorder; I h; NAc; serotonin; I DA; dopamine D 2 receptor-mediated GIRK current; tipepidine; G protein-coupled inwardly rectifying potassium channel; noradrenaline; TritonX-100 in TBS; I Baclofen; patch clamp; inhibitory postsynaptic currents; TBS; noradrenaline transporter; TBST; SNc; I DA(GIRK); nucleus accumbens; S.E.M; tyrosine hydroxylase; dopamine-induced current; hyperpolarization-activated cation current; OCD; substantia nigra pars compacta; PB; SERT; NA; ventral tegmental area; TH; serotonin transporter; IPSCs; G-protein-coupled inwardly rectifying potassium; GPCR; tris-buffered saline; GIRK; standard error of the mean; 5-HT; dopamine; DA; IDA; IDA(GIRK); IBaclofen; Ih; Dopamine; Tipepidine; Central nervous system; Electrophysiology; Patch clamp method; Ionic channel; Catecholamine; Encephalon; D2 Dopamine receptor; Neurotransmitter; Dopaminergic neuron; Ventral tegmental area; Inward current; Potassium; G protein; I(Baclofen); I(h); I(DA(GIRK)); dopamine D receptor-mediated GIRK current; I(DA)
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2013.07.044

Highlights • Tipepidine inhibited DA D2 receptor-mediated GIRK channel currents in VTA neurons. • Tipepidine activated single VTA neurons inhibited by dopamine. • Tipepidine increased double immunopositive cells for TH and c-Fos in VTA. • The results show a part of mechanisms for novel antidepressant effect of tipepidine.