Mass spectral evidence of the occurrence of vindoline in heterotrophic cultures of Catharanthus roseus cells

Vindoline concentrations in the leaves of 70 CATHARANTHUS ROSEUS of 3 cultivars were analyzed by HPLC, and 3 plants were selected for starting callus cultures on different media. When the initial calli were analyzed using a vindoline-specific RIA, the assay suggested a vindoline content of about 10...

Full description

Saved in:
Bibliographic Details
Published inPlanta medica Vol. 55; no. 2; pp. 155 - 157
Main Authors Naaranlahti, T, Lapinjoki, S.P, Huhtikangas, A, Toiwonen, L, Kurten, U, Kauppinen, V, Lounasmaa, M
Format Journal Article
LanguageEnglish
Published Germany 01.04.1989
Subjects
alkaloids
Catharanthus roseus
cell culture
indoles
medicinal properties
Online AccessGet more information

Cover

More Information
Summary:Vindoline concentrations in the leaves of 70 CATHARANTHUS ROSEUS of 3 cultivars were analyzed by HPLC, and 3 plants were selected for starting callus cultures on different media. When the initial calli were analyzed using a vindoline-specific RIA, the assay suggested a vindoline content of about 10 (-5)% dry weight for one-third of the first 60 cultures examined. Due to the unexpectedly high incidence of vindoline-positive calli, the screening programme was discontinued and efforts were concentrated on verifying the existence of this alkaloid in the cells. Suspension cultures derived from the 5 most immunopositive calli in an alkaloid production medium were analyzed by HPLC and GC/MS. Comparison with reference material showed that the heterotrophic suspension cultures contained a compound identical to vindoline.
ISSN:0032-0943
1439-0221
DOI:10.1055/s-2006-961911