Short communication: Effect of commercial or depurinized milk diet on plasma advanced oxidation protein products, cardiovascular markers, and bone marrow CD34+ stem cell potential in rat experimental hyperuricemia

• Published in: Journal of dairy science Vol. 97; no. 11; pp. 6823 - 6827
• Main Authors: Kocic, Gordana, Sokolovic, Dusan, Jevtovic, Tatjana, Cvetkovic, Tatjana, Veljkovic, Andrej, Kocic, Hristina, Stojanovic, Svetlana, Jovanovic, Aneta, Jovanovic, Jelena, Zivkovic, Petar
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2014
• Subjects: advanced oxidation protein products (AOPP); Advanced Oxidation Protein Products - blood; Allopurinol - therapeutic use; Animals; Antigens, CD34 - metabolism; Biomarkers - blood; Bone Marrow - physiology; bone marrow CD34; cardiovascular marker; Disease Models, Animal; hyperuricemia; Hyperuricemia - diet therapy; Hyperuricemia - metabolism; Hyperuricemia - pathology; Lipoproteins, HDL - blood; milk; Milk - chemistry; Oxidative Stress; Purines - analysis; Rats; Stem Cells - physiology; Uric Acid - analysis; milk; advanced oxidation protein products (AOPP); hyperuricemia; cardiovascular marker; bone marrow CD34; bone marrow CD34(+)
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2014-8556

Cardiovascular repair and myocardial contractility may be improved by migration of bone marrow stem cells (BMSC) and their delivery to the site of injury, a process known as BMSC homing. The aim of our study was to examine the dietary effect of a newly patented depurinized milk (DP) that is almost free of uric acid and purine and pyrimidine compounds compared with a standard commercial 1.5% fat UHT milk diet or allopurinol therapy in rat experimental hyperuricemia. Bone marrow stem cell potential (BMCD34+, CD34-postive bone marrow cells), plasma oxidative stress parameters [advanced oxidation protein products, AOPP) and thiobarbituric acid reactive substances (TBARS)], myocardial damage markers [creatine phosphokinase (CPK), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH)], plasma cholesterol, and high-density lipoprotein cholesterol were investigated. The DP milk diet significantly increased the number of BMCD34+ stem cells compared with commercial UHT milk. Allopurinol given alone also increased the number of BMCD34+. Hyperuricemia caused a significant increase in all plasma enzyme markers for myocardial damage (CPK, LDH, and AST). A cardioprotective effect was achieved with allopurinol but almost equally with DP milk and more than with commercial milk. Regarding plasma AOPP, TBARS, and cholesterol levels, the most effective treatment was DP milk. In conclusion, the protective role of a milk diet on cardiovascular function may be enhanced through the new depurinized milk diet, which may improve cardiovascular system function via increased bone marrow stem cell regenerative potential, decreased plasma oxidative stress parameters, and decreased levels of myocardial damage markers and cholesterol. New dairy technology strategies focused on eliminating harmful milk compounds should be completely nontoxic. Novel milk products should be tested for their ability to improve tissue repair and function.