Isolation and expression studies of the ERD15 gene involved in drought-stressed responses

• Published in: Genetics and molecular research Vol. 13; no. 4; pp. 10852 - 10862
• Main Authors: Shao, H H, Chen, S D, Zhang, K, Cao, Q H, Zhou, H, Ma, Q Q, He, B, Yuan, X H, Wang, Y, Chen, Y H, Yong, B
• Format: Journal Article
• Language: English
• Published: Brazil 19.12.2014
• Subjects: Cloning, Molecular - methods; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Ipomoea batatas - genetics; Ipomoea batatas - physiology; Phylogeny; Plant Proteins - genetics; Polyethylene Glycols - pharmacology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Sequence Alignment; Sequence Analysis, DNA; Solanum tuberosum; Stress, Physiological
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2014.December.19.6

The early response to the dehydration 15 (ERD15) gene is widely involved in the processes of signal transduction, programmed cell death, gene transcription, and stress tolerance in plants. In a previous study, the ERD15 gene was shown to be an important regulator of the abscisic acid response and salicylic acid-dependent defense pathway, acting as an important negative regulator of abscisic acid. The complete IbERD15 gene (accession No. KF723428) was isolated by reverse transcription-polymerase chain reaction. The IbERD15 gene contains an open reading frame of 504 bp, encodes a peptide of 167 amino acids, and has a molecular mass of 18.725 kDa. The transcript levels of the IbERD15 gene in a variety of tissues were examined by digital gene expression profiling. The roots of the sweet potato were treated by 3 degrees of polyethylene glycol, and the results indicate that the IbERD15 gene might play an important role in the defense response to drought stress. Moreover, the IbERD15 gene was successfully transformed into yeast cells for analysis of drought tolerance in transgenic yeast.