The Epigenetic Reprogramming Roadmap in Generation of iPSCs from Somatic Cells

Reprogramming of somatic cells to induced pluripotent stem cells （iPSCs） is a comprehensive epigenetic process involving genome-wide modifications of histones and DNA methylation. This process is often incomplete, which subsequently affects iPSC reprograming, pluripotency, and differentiation capaci...

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Bibliographic Details
Published inJournal of genetics and genomics Vol. 42; no. 12; pp. 661 - 670
Main Authors Brix, Jacob, Zhou, Yan, Luo, Yonglun
Format Journal Article
LanguageEnglish
Published China Elsevier Ltd 20.12.2015
Subjects
Animals
Cellular Reprogramming
DNA Methylation
DNA修饰
DNA甲基化
Epigenetic memory
Epigenetics
Epigenomics
Histone modification
Histones - genetics
Histones - metabolism
Humans
Induced pluripotent stem cells (iPSCs)
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Reprogramming
体细胞
组蛋白修饰
表观遗传
路线图
重新编程
重编程
Epigenetics
DNA methylation
Histone modification
Reprogramming
Epigenetic memory
Induced pluripotent stem cells (iPSCs)
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Summary:Reprogramming of somatic cells to induced pluripotent stem cells （iPSCs） is a comprehensive epigenetic process involving genome-wide modifications of histones and DNA methylation. This process is often incomplete, which subsequently affects iPSC reprograming, pluripotency, and differentiation capacity. Here, we review the epigenetic changes with a focus on histone modification （methylation and acetylation） and DNA modification （methylation） during iPSC induction. We look at changes in specific epigenetic signatures, aberrations and epigenetic memory during reprogramming and small molecules influencing the epigenetic reprogramming of somatic cells. Finally, we discuss how to improve iPSC generation and pluripotency through epigenetic manipulations.
Bibliography:Reprogramming of somatic cells to induced pluripotent stem cells （iPSCs） is a comprehensive epigenetic process involving genome-wide modifications of histones and DNA methylation. This process is often incomplete, which subsequently affects iPSC reprograming, pluripotency, and differentiation capacity. Here, we review the epigenetic changes with a focus on histone modification （methylation and acetylation） and DNA modification （methylation） during iPSC induction. We look at changes in specific epigenetic signatures, aberrations and epigenetic memory during reprogramming and small molecules influencing the epigenetic reprogramming of somatic cells. Finally, we discuss how to improve iPSC generation and pluripotency through epigenetic manipulations.
Induced pluripotent stem cells （iPSCs）; Reprogramming; Epigenetics; Histone modification; DNA methylation; Epigenetic memory
11-5450/R
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ISSN:1673-8527
DOI:10.1016/j.jgg.2015.10.001