Non-association of Crohn's disease with NOD2 gene variants in Moroccan patients

• Published in: Gene Vol. 499; no. 1; pp. 121 - 123
• Main Authors: Hama, I., Ratbi, I., Reggoug, S., Elkerch, F., Kharrasse, G., Errabih, I., Ouazzani, H., Sefiani, A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10.05.2012
• Subjects: Adolescent; Adult; alleles; Case-Control Studies; chronic diseases; Crohn disease; Crohn Disease - epidemiology; Crohn Disease - genetics; Crohn's disease; Female; gastrointestinal system; gene frequency; Genetic Association Studies; Genetics, Population; genotype; genotyping; Humans; innate immunity; intracellular signaling peptides and proteins; Male; meta-analysis; Middle Aged; Moroccan population; Morocco - epidemiology; Nod2 Signaling Adaptor Protein - genetics; NOD2/CARD15; patients; Pilot Projects; Polymorphism, Genetic - physiology; single nucleotide polymorphism; Variant; Young Adult; Morocco; NOD2/CARD15; Variant; CD; OR; CI; Moroccan population; IBD; Crohn's disease; NOD2; PCR
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2011.12.045

Crohn's disease is a chronic inflammatory bowel disease, with multifactorial traits, that can involve any part of the gastrointestinal tract. In recent years, a dozen genome-wide association scan and meta-analysis were published bringing the number of susceptibility alleles to more than 30 variations. However, the major susceptibility gene for Crohn's disease is NOD2, located on proximal 16q, which is involved in the innate immune response. Three main variants of this gene: two single nucleotide polymorphisms p.Arg702Trp and p.Gly908Arg substitutions and frameshift polymorphism p.Leu1007fsinsC are involved in susceptibility to Crohn's disease. There is no data about the frequency of these allelic variants in Moroccan patients with Crohn's disease. The aim of our study is to genotype the NOD2 gene to assess the involvement of these three variants in susceptibility to Crohn's disease for Moroccans. We carried out genotyping for the three variants p.Arg702Trp, p.Gly908Arg and p.Leu1007fsinsC of NOD2 gene using PCR-sequencing among 101 Moroccan patients with Crohn's disease and 107 healthy controls. The three main variants of NOD2 gene were present in Moroccan patients with no significant difference compared to controls. This preliminary study shows no evidence association of NOD2 gene with Crohn's disease in the Moroccan population. ► We recruited a group of Moroccan patients with Crohn’s disease, and a control group. ► We genotyped three major variants of the NOD2 gene in the two groups. ► We compared the genotype frequencies between the two groups by statistical test. ► The three variants are not involved in Crohn’s disease susceptibility in Moroccans.