PIM1 controls GBP1 activity to limit self-damage and to guard against pathogen infection

Disruption of cellular activities by pathogen virulence factors can trigger innate immune responses. Interferon-γ (IFN-γ)-inducible antimicrobial factors, such as the guanylate binding proteins (GBPs), promote cell-intrinsic defense by attacking intracellular pathogens and by inducing programmed cel...

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Published inScience (American Association for the Advancement of Science) Vol. 382; no. 6666; p. eadg2253
Main Authors Fisch, Daniel, Pfleiderer, Moritz M, Anastasakou, Eleni, Mackie, Gillian M, Wendt, Fabian, Liu, Xiangyang, Clough, Barbara, Lara-Reyna, Samuel, Encheva, Vesela, Snijders, Ambrosius P, Bando, Hironori, Yamamoto, Masahiro, Beggs, Andrew D, Mercer, Jason, Shenoy, Avinash R, Wollscheid, Bernd, Maslowski, Kendle M, Galej, Wojtek P, Frickel, Eva-Maria
Format Journal Article
LanguageEnglish
Published United States The American Association for the Advancement of Science 06.10.2023
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Summary:Disruption of cellular activities by pathogen virulence factors can trigger innate immune responses. Interferon-γ (IFN-γ)-inducible antimicrobial factors, such as the guanylate binding proteins (GBPs), promote cell-intrinsic defense by attacking intracellular pathogens and by inducing programmed cell death. Working in human macrophages, we discovered that GBP1 expression in the absence of IFN-γ killed the cells and induced Golgi fragmentation. IFN-γ exposure improved macrophage survival through the activity of the kinase PIM1. PIM1 phosphorylated GBP1, leading to its sequestration by 14-3-3σ, which thereby prevented GBP1 membrane association. During infection, the virulence protein TgIST interfered with IFN-γ signaling and depleted PIM1, thereby increasing GBP1 activity. Although infected cells can restrain pathogens in a GBP1-dependent manner, this mechanism can protect uninfected bystander cells. Thus, PIM1 can provide a bait for pathogen virulence factors, guarding the integrity of IFN-γ signaling.
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content type line 23
ISSN:0036-8075
1095-9203
1095-9203
DOI:10.1126/science.adg2253