Hyaluronic acid production by recombinant Lactococcus lactis

• Published in: Applied microbiology and biotechnology Vol. 77; no. 2; pp. 339 - 346
• Main Authors: Chien, Liang-Jung, Lee, Cheng-Kang
• Format: Journal Article
• Language: English
• Published: Berlin Berlin/Heidelberg : Springer-Verlag 01.11.2007; Springer; Springer Nature B.V
• Subjects: Acid production; Bacteria; Binding sites; Biological and medical sciences; Biotechnology; Biotechnology - methods; Chromosomes; Cloning; Dehydrogenases; Deoxyribonucleic acid; DNA; E coli; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Bacterial; Genetic engineering; Genetics; Glucose; Glucuronosyltransferase - genetics; Glucuronosyltransferase - metabolism; HA synthase expression; Hyaluronic acid; Hyaluronic Acid - biosynthesis; Lactococcus lactis; Lactococcus lactis - enzymology; Lactococcus lactis - genetics; Microorganisms; NICE; nisin; Nisin - pharmacology; Pathogens; Plasmids; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Streptococcus - enzymology; Streptococcus - genetics; Studies; Uridine Diphosphate Glucose Dehydrogenase - genetics; Uridine Diphosphate Glucose Dehydrogenase - metabolism; Streptococcaceae; Lactococcus lactis; Lactic acid bacteria; Production; Bacteria; Micrococcales; Hyaluronic acid; Genetically modified microorganism
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-007-1153-z

Microbial hyaluronic acid (HA), commonly produced by pathogenic Streptococcus, was made possible to be produced by a generally recognized as safe Lactococcus lactis by coexpressing HA synthase and uridine diphosphate-glucose dehydrogenase (UDP-GlcDH) of Streptococcus equi subsp. zooepidemicus in a nisin-controlled expression (NICE) system. With scarce expressed HA synthase alone, the constructed recombinant L. lactis (LL-NA) strain could produce HA with a concentration about 0.08 g/l in the M17 medium supplemented with 1% (w/v) glucose. In contrast to HA synthase, UDP-GlcDH of Streptococcus could be overexpressed in the NICE system. With coexpression of heterologous UDP-GlcDH with HA synthase, the constructed LL-NAB strain grew slightly slower to a concentration about 10% lower that of the LL-NA strain. However, the HA concentration produced was enhanced about eightfold to 0.65 g/l.