Linear Remodeling of Helical Virus by Movement Protein Binding

• Published in: Journal of molecular biology Vol. 333; no. 3; pp. 565 - 572
• Main Authors: Rodionova, Nina P., Karpova, Olga V., Kozlovsky, Stanislav V., Zayakina, Olga V., Arkhipenko, Marina V., Atabekov, Joseph G.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 24.10.2003
• Subjects: Capsid Proteins - metabolism; disassembly; linear destabilization; Microscopy, Electron; movement protein; Plant Viral Movement Proteins; potato virus X; Potexvirus - chemistry; Potexvirus - genetics; Potexvirus - metabolism; Potexvirus - ultrastructure; Protein Binding; Protein Biosynthesis; Protein Structure, Secondary; RNA, Viral - metabolism; RNA-Binding Proteins - metabolism; translational activation; Viral Nonstructural Proteins - metabolism; Viral Proteins - metabolism; Virus Assembly; linear destabilization; WGE, wheat germ extract; disassembly; translational activation; movement protein; PVX, potato virus X; CP, coat protein; TGB, triple gene block; potato virus X
• ISSN: 0022-2836; 1089-8638
• DOI: 10.1016/j.jmb.2003.08.058

Previously we have shown that encapsidated potato virus X (PVX) RNA was nontranslatable in vitro, but could be converted into a translatable form by binding of the PVX-coded movement protein (termed TGBp1) to one end of a polar helical PVX virion. We reported that binding of TGBp1 to coat protein (CP) subunits located at one extremity of the helical particles induced a linear destabilization of the CP helix, which was transmitted along the whole particle. Two model structures were used: (i) native PVX and (ii) artificial polar helical PVX-like particles lacking intact RNA (PVX RNA-DEG). Binding of TGBp1 to the end of either of these particles led to their destabilization, but no disassembly of the CP helix occurred. Influence of additional factors was required to trigger rapid disassembly of TGBp1-PVX and TGBp1-PVX RNA-DEG complexes. Thus: (i) no disassembly was observed unless TGBp1-PVX complex was translated. A novel phenomenon of TGBp1-dependent, ribosome-triggered disassembly of PVX was described: initiation of translation and few translocation steps were needed to trigger rapid (and presumably cooperative) disassembly of TGBp1-PVX into protein subunits and RNA. Importantly, the whole of the RNA molecule (including its 3′-terminal region) was released. The TGBp1-induced linear destabilization of CP helix was reversible, suggesting that PVX in TGBp1-PVX complex was metastable; (ii) entire disassembly of the TGBp1-PVX RNA-DEG complex (but not of the TGBp1-free PVX RNA-DEG particles) into 2.8 S subunits was triggered under influence of a centrifugal field. To our knowledge, transmission of the linear destabilization along the polar helical protein array induced by a foreign protein binding to the end of the helix represents a novel phenomenon. It is tempting to suggest that binding of TGBp1 to the end of the PVX CP helix induced conformational changes in terminal CP subunits that can be linearly transferred along the whole helical particle, i.e. that intersubunit conformational changes may be transferred along the CP helix.