Modification research on in wall of capillary copper tube with Norland optical adhesive 68 in a double stereo PCR microfluidic chip

• Published in: Genetics and molecular research Vol. 14; no. 4; pp. 13603 - 13611
• Main Authors: Wu, J, Wang, F J, Wang, C Y, Yu, K X, Ma, Y, Chen, T, Li, Y H, Zheng, Y
• Format: Journal Article
• Language: English
• Published: Brazil 01.01.2015
• Subjects: Copper - chemistry; Microfluidics - instrumentation; Microfluidics - methods; Polymerase Chain Reaction - instrumentation; Polymerase Chain Reaction - methods
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2015.October.28.21

In this study, a Norland optical adhesive 68 (NOA68) film, approximately 2.2 μm thick, was manufactured using ultraviolet solidified NOA68 in apparatus manufacturing film on the inwall of a capillary copper pipe, developed in our laboratory. The roughness of the inwall of capillary copper pipe was improved from Ra = 0.766 to 0.204 μm and the contact angle was improved from approximately 96° to 55°, increasing hydrophilicity. Polymerase chain reaction experiments indicated that the ratio of work pressure in the microfluidic chip before modification to that after modification was 2.71/1, indicating that the extension efficiency increased. Our results provide a basis for the construction of a microform chip based on function integration.