An accurate, high-throughput method for genotyping the −373AnTn polymorphism in the interleukin-6 promoter

Summary Interleukin‐6 (IL‐6) is a pro‐inflammatory cytokine implicated in inflammatory diseases. The four promoter polymorphisms (−174G/C, −373AnTn, −572G/C, −597G/A) have been shown to function as a haplotype in determining IL‐6 gene transcription. Using a capillary‐based electrophoresis system, hi...

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Bibliographic Details
Published inInternational Journal of Immunogenetics Vol. 33; no. 2; pp. 65 - 67
Main Authors Khwaja, Haris A., Green, Fiona R.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.04.2006
Subjects
Adenine
Electrophoresis, Capillary
Genotype
Humans
Interleukin-6 - genetics
Polymorphism, Genetic
Promoter Regions, Genetic - genetics
Sequence Analysis, DNA - methods
Sequence Analysis, DNA - standards
Thymine
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Summary:Summary Interleukin‐6 (IL‐6) is a pro‐inflammatory cytokine implicated in inflammatory diseases. The four promoter polymorphisms (−174G/C, −373AnTn, −572G/C, −597G/A) have been shown to function as a haplotype in determining IL‐6 gene transcription. Using a capillary‐based electrophoresis system, high‐throughput genotyping for the −373AnTn polymorphism was achieved, thus facilitating IL‐6 promoter haplotype determination.
Bibliography:ark:/67375/WNG-S4QRCT3N-X
istex:98DCCA942F9E9018A48179D574B89840F1865EEA
ArticleID:IJI569
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1744-3121
1744-313X
1365-2370
DOI:10.1111/j.1744-313X.2006.00569.x