Role of de‐N‐acetylase PgaB from Aggregatibacter actinomycetemcomitans in exopolysaccharide export in biofilm mode of growth

Summary Aggregatibacter actinomycetemcomitans, a Gram‐negative bacterium, is the causative agent of localized aggressive periodontitis. Attachment to a biotic surface is a critical first step in the A. actinomycetemcomitans infection process for which exopolysaccharides have been shown to be essenti...

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Published inMolecular oral microbiology Vol. 32; no. 6; pp. 500 - 510
Main Authors Shanmugam, M, Oyeniyi, A.O, Parthiban, C, Gujjarlapudi, S.K, Pier, G.B, Ramasubbu, N
Format Journal Article
LanguageEnglish
Published Denmark 01.12.2017
Subjects
biofilm
deacetylation
Dentistry
exopolysaccharide
export
oral pathogen
virulence
exopolysaccharide
oral pathogen
virulence
export
deacetylation
biofilm
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Summary:Summary Aggregatibacter actinomycetemcomitans, a Gram‐negative bacterium, is the causative agent of localized aggressive periodontitis. Attachment to a biotic surface is a critical first step in the A. actinomycetemcomitans infection process for which exopolysaccharides have been shown to be essential. In addition, the pga operon, containing genes encoding for biosynthetic proteins for poly‐N‐acetyl glucosamine (PNAG), plays a key role in A. actinomycetemcomitans virulence, as a mutant strain lacking the pga operon induces significantly less bone resorption. Among the genes in the pga operon, pgaB codes for a de‐N‐acetylase that is responsible for the deacetylation of the PNAG exopolysaccharide. Here we report the role of PgaB in regulation of virulence genes using a markerless, scarless deletion mutant targeting the coding region of the N‐terminal catalytic domain of PgaB. The results demonstrate that the N‐terminal, catalytic domain of PgaB is crucial for exopolysaccharide export.
Bibliography:Funding information
This work was supported by the United States Public Health Service, National Institutes of Health, National Institute of Dental and Craniofacial Research DE022544 (N.R.).
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ISSN:2041-1006
2041-1014
DOI:10.1111/omi.12188