Microsurgical cloning techniques for zygotes: a long-term dispute

Abstract Zygotes (fertilized oocytes) have been considerably neglected as suitable recipients for cell nuclear transfer in cloning. Recently, it has been reported by Polish researchers that microsurgical methods using very thin pipettes for pronuclear removal from mouse zygotes seem to be crucial fo...

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Bibliographic Details
Published inReproductive biomedicine online Vol. 18; no. 1; pp. 7 - 8
Main Author Illmensee, Karl
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Ltd 2009
Subjects
Animals
cloning
Cloning, Organism - methods
Consensus
Cumulus Cells - cytology
cytoplasm
Mice
Microsurgery - instrumentation
Microsurgery - methods
Needles
nuclear transfer
Nuclear Transfer Techniques - instrumentation
nuclei
Obstetrics and Gynecology
oocyte
pronuclear chromatin
pronucleus
Zygote - cytology
Zygote - physiology
cytoplasm
nuclei
pronucleus
oocyte
pronuclear chromatin
nuclear transfer
cloning
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Summary:Abstract Zygotes (fertilized oocytes) have been considerably neglected as suitable recipients for cell nuclear transfer in cloning. Recently, it has been reported by Polish researchers that microsurgical methods using very thin pipettes for pronuclear removal from mouse zygotes seem to be crucial for successful cloning of adult mice. It was postulated that due to this technique both pronuclei are broken, leaving behind some nuclear components in the oocyte's cytoplasm. Release of pronuclear factors into the cytoplasm turns enucleated zygotes into suitable nuclear recipients for cloning.
Bibliography:ObjectType-Article-1
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ISSN:1472-6483
1472-6491
DOI:10.1016/S1472-6483(10)60417-8