High glucose promotes the CTGF expression in human mesangial cells via serum and glucocorticoid-induced kinase 1 pathway

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 28; no. 5; pp. 508 - 512
• Main Authors: Wang, Quansheng, Zhang, Ali, Li, Renkang, Liu, Jianguo, Xie, Jiwen, Deng, Anguo, Feng, Yuxi, Zhu, Zhonghua
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.10.2008; Department of Integrated Chinese Medicine and Western Medicine,Wuhan 430022, China%Department of Radiotherapy and Chemotherapy, Zhongnan Hospital Wuhan University, Wuhan 430071, China%Department of Nephrology, Union Hospital TongJi Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
• Subjects: Cells, Cultured; Connective Tissue Growth Factor - genetics; Connective Tissue Growth Factor - metabolism; Glucose - pharmacology; Humans; Immediate-Early Proteins - metabolism; Immediate-Early Proteins - physiology; Medicine; Medicine & Public Health; Mesangial Cells - cytology; Mesangial Cells - metabolism; Protein-Serine-Threonine Kinases - metabolism; Protein-Serine-Threonine Kinases - physiology; Signal Transduction - drug effects; connective tissue growth factor; serum and glucocorticoid-induced protein kinase 1; human mesangial cells; high glucose; diabetic nephropathy; human mesahgial cells
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-008-0504-z

Summary The role of serum and glucocorticoid-induced kinase 1 (SGK1) pathway in the connective tissue growth factor (CTGF) expression was investigated in cultured human mesangial cells (HMCs) under high glucose. By using RT-PCR and Western blot, the effect of SGK1 on the CTGF expression in HMCs under high glucose was examined. Overexpression of active SGK1 in HMCs transfected with p IRES 2 -EGFP- S422D hSGK1 (SD) could increase the expression of phosphorylated SGK1 and CTGF as compared with HMCs groups transfected with p IRES 2 -EGFP (FP) under high glucose or normal glucose. Overexpression of inactive SGK1 in HMCs transfected with p IRES 2 -EGFP- K127N hSGK1 (KN) could decrease phosphorylated SGK1 and CTGF expression as compared with HMCs groups transfected with FP under high glucose. In conclusion, these results suggest that high glucose-induced CTGF expression is mediated through the active SGK1 in HMCs.