Polyclonal antibodies specific for VP1 and VP3 capsid proteins of taura syndrome virus (TSV) produced via gene cloning and expression

• Published in: Diseases of aquatic organisms Vol. 69; no. 2-3; pp. 249 - 253
• Main Authors: CHAIVISUTHANGKURA, Parin, TEJANGKURA, Thanawan, RUKPRATANPORN, Sombat, LONGYANT, Siwaporn, SITHIGORNGUL, Weerawan, SITHIGORNGUL, Paisarn
• Format: Journal Article
• Language: English
• Published: Oldendorf Inter-Research 06.04.2006
• Subjects: Animal aquaculture; Animal productions; Animals; Antibodies, Viral - biosynthesis; Biological and medical sciences; Blotting, Western - methods; Capsid Proteins - genetics; Capsid Proteins - immunology; Cloning, Molecular - methods; Crustacea; DNA Primers - chemistry; Escherichia coli; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Immune Sera - immunology; Immunohistochemistry - veterinary; Invertebrate aquaculture; Mice; Penaeidae - virology; Recombinant Proteins - biosynthesis; Recombinant Proteins - immunology; RNA Viruses - genetics; RNA Viruses - immunology; RNA Viruses - isolation & purification; Taura syndrome virus; Immunohistochemistry; Western blot; Antibody; Polyclonal antibody; Zoopathogen; Gene expression; Protein; Macrura; Crustacea; Marine environment; Virus; Penaeidae; Edible crustacean; Arthropoda; Pathogenic; TSV; VP1; VP3; Decapoda; Litopenaeus vannamei; Invertebrata; Capsid; Aquaculture; Taura syndrome virus
• ISSN: 0177-5103; 1616-1580
• DOI: 10.3354/dao069249

Capsid protein genes VP1 and VP3 of Taura syndrome virus (TSV) were cloned into pGEX-6P-1 expression vector and transformed into Escherichia coli BL21. After induction, recombinant VP1 (rVP1) and recombinant VP3 (rVP3) were produced, purified by SDS-PAGE and used for immunization of Swiss mice for antisera production. Anti-rVP1 and anti-rVP3 antisera showed specific immunoreactivities to rVP1 and rVP3 proteins, respectively, by Western blot assay and also yielded good results for detection of TSV in various shrimp tissues by immunohistochemistry. This is the first step towards our target of preparing monoclonal antibodies specific to rVP1 and rVP3 for use in simple immuno-diagnostic test kits for TSV detection and identification.