Molecular characterisation of an Australian isolate of Bonamia exitiosa

• Published in: Diseases of aquatic organisms Vol. 71; no. 1; pp. 81 - 85
• Main Authors: CORBEIL, Serge, ARZUL, Isabelle, ROBERT, Maeva, BERTHE, Franck C. J, BESNARD-COCHENNEC, Nathalie, CRANE, Mark St. J
• Format: Journal Article
• Language: English
• Published: Oldendorf Inter-Research 11.07.2006
• Subjects: Animals; Base Sequence; Biological and medical sciences; Bonamia; Bonamia exitiosa; DNA Primers - chemistry; DNA, Protozoan - chemistry; DNA, Protozoan - isolation & purification; DNA, Ribosomal Spacer - genetics; Fundamental and applied biological sciences. Psychology; Haplosporida - genetics; Haplosporida - isolation & purification; Invertebrates; Marine; Molecular Sequence Data; Mollusca; New South Wales; Ostrea - parasitology; Ostrea angasi; Pathology; Protozoa; RNA, Ribosomal, 18S - genetics; Sequence Alignment; Systematics. Geographical distribution. Morphology. Cytology; New South Wales; Australia; France; Europe; Oceania; PSE, Australia, New South Wales; Protozoa; Apicomplexa; Edible mollusc; Parasite; Zoopathogen; Subunit; Ostrea angasi; Small subunit rRNA operon; Geographic distribution; Flat oyster; Bivalvia; Ribosomal RNA; Bonamia species; Pathogenic; Specific identification; Molecular systematics; Invertebrata; Mollusca
• ISSN: 0177-5103; 1616-1580
• DOI: 10.3354/dao071081

An Australian (New South Wales) isolate of Bonamia was characterised at the molecular level by sequencing the 18S-ITS-1 region of the small subunit rRNA operon obtained from flat oysters Ostrea angasi shown to be infected by histological examination. Sequence data alignment with homologous genes from 2 other isolates of Bonamia (New Zealand and France) revealed high levels of nucleotide identity with both isolates. However, the Australian Bonamia is shown to be more closely related to the New Zealand isolate, suggesting the existence of an oceanic subgroup of Bonamia.