Correlation of contractile force with a calcium pool in the isolated cat heart

The relationship between cellular calcium (Ca) stores and isometric contractile force was investigated in isolated, gas-perfused cat hearts. The hearts were preperfused for approximately 5 min with substrate-free Krebs solution modified to contain 0, 1.25, 2.5, 5.0 or 10.0 mEq/liter Ca, gas-perfused...

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Bibliographic Details
Published inThe Journal of general physiology Vol. 52; no. 6; pp. 969 - 982
Main Authors Bailey, L E, Dresel, P E
Format Journal Article
LanguageEnglish
Published United States The Rockefeller University Press 01.12.1968
Subjects
Animals
Calcium - analysis
Calcium - pharmacology
Calcium Isotopes
Cats
Heart - physiology
Kinetics
Methods
Muscle Contraction - drug effects
Muscles - physiology
Myocardium - metabolism
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Summary:The relationship between cellular calcium (Ca) stores and isometric contractile force was investigated in isolated, gas-perfused cat hearts. The hearts were preperfused for approximately 5 min with substrate-free Krebs solution modified to contain 0, 1.25, 2.5, 5.0 or 10.0 mEq/liter Ca, gas-perfused for up to 120 min, and then perfused with zero-Ca Krebs solution. Contractile force and [Ca] in the effluent fluid were measured. Our results indicate that: (a) The washout of Ca was characteristic of a three compartment system, (b) A Ca compartment directly associated with muscle contraction was identified by correlation of the rate of Ca washout with the rate of decay of contractility (r = 0.79). (c) Ca content in the compartment was correlated with contractile force (r = 0.77). (d) Contractile force and the Ca content of the compartment which was correlated with contractility approached a steady state during 120 min of gas perfusion.
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ISSN:0022-1295
1540-7748
DOI:10.1085/jgp.52.6.969