The molecular regulatory mechanism of reed canary grass under salt, waterlogging, and combined stress was analyzed by transcriptomic analysis

• Published in: BMC plant biology Vol. 24; no. 1; pp. 857 - 13
• Main Authors: Jia, Xuejie, Xiong, Yi, Xiong, Yanli, Li, Daxu, Ji, Xiaofei, Lei, Xiong, You, Minghong, Bai, Shiqie, Zhang, Jianbo, Ma, Xiao
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 13.09.2024; BioMed Central; BMC
• Subjects: Abiotic stress; Agricultural production; Anatomical structure; Botanical research; China; Climate change; Combined stress; Cultivars; Dehydrogenases; Energy conversion efficiency; Energy metabolism; Environmental aspects; Environmental restoration; Floods; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genetic aspects; Glutathione; Grasses; High altitude; Leaves; Metabolic pathways; Na+/K+-exchanging ATPase; Phalaris - genetics; Phalaris - metabolism; Phalaris - physiology; Phalaris arundinacea; Physiological aspects; Physiology; Plant breeding; Plant genetics; Poaceae - genetics; Poaceae - metabolism; Poaceae - physiology; Reed canary grass; Regulatory mechanisms (biology); Respiration; Salinity; Salt stress (Botany); Salt Stress - genetics; Salts; Sequence analysis; Signal transduction; Software; Stress (Physiology); Stress, Physiological - genetics; Transcriptome; Transcriptomes; Transcriptomics; Waterlogging; Wetlands; China; Tibetan Plateau; United States > US; Europe; Sichuan China; Reed canary grass; Transcriptomics; Anatomical structure; Combined stress
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05564-w

Reed canary grass has been identified as a suitable species for restoring plateau wetlands and understanding plant adaptation mechanisms in wetland environments. In this study, we subjected a reed canary grass cultivar 'Chuanxi' to waterlogging, salt, and combined stresses to investigate its phenotypic characteristics, physiological indices, and transcriptome changes under these conditions. The results revealed that the growth rate was slower under salt stress than under waterlogging stress. The chlorophyll content and energy capture efficiency of the PS II reaction center decreased with prolonged exposure to each stress. Conversely, while the activities of enzymes associated with respiratory metabolism, as well as MDA, PRO, Na , and K -ATPase, increased. The formation of distinct aerenchyma was observed under waterlogging stress and combined stress. Transcriptome sequencing analysis identified 5,379, 4,169, and 14,993 DEGs under CK vs. W, CK vs. S, and CK vs. SW conditions, respectively. The WRKY was found to be the most abundant under waterlogging stress, whereas the MYB predominated under salt stress and combined stress. Glutathione metabolic pathways and Plant hormone signal transduction have also been found to play important roles in stress. By integrating phenotypic, physiological, anatomical, and transcriptomic, this research provides valuable insights into how reed canary grass responds to salt, waterlogging, and combined stresses. These findings may inform the ecological application of reed canary grass in high-altitude wetlands and for breeding purposes.