Preparation and certification of a fish oil natural matrix reference material for organochlorine pesticides

• Published in: Accreditation and quality assurance Vol. 15; no. 10; pp. 563 - 568
• Main Authors: Shehata, Adel B., Tahoun, Ibrahim F.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.10.2010; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Applied sciences; Benzene; Biochemistry; Calibration; Certification; Chemistry; Chemistry and Materials Science; Chlorobenzene; Chromatographic methods and physical methods associated with chromatography; Chromatography; Commercial Law; Ecotoxicology; Exact sciences and technology; Fish oils; Food Science; Freshwater; Gas chromatographic methods; Global environmental pollution; Marketing; Organic contaminants; PCB; Pesticides; Pollution; Polychlorinated biphenyls; Practitioner's Report; Reference materials; Sodium; Tilapia; Germany; Homogeneity; GC; Fish oil; Combining data; Pesticides; Uncertainty; Chemical analysis; Use; Standard; Certified reference material; Certification; Gas chromatography; Vertebrata; Ethylbenzene; Analysis method; Sample preparation; DDT; Organochlorine compounds; Pisces; Analytical method; Reference material; Environment
• ISSN: 0949-1775; 1432-0517
• DOI: 10.1007/s00769-010-0678-5

The mass fractions of six organochlorine pesticides in a fish oil certified reference material (CRM) have been determined using multiple methods of analysis. Fish oil was extracted from the filet of Tilapia fish collected from the River Nile, and this CRM was recently issued by the National Institute of Standards (NIS). It can be used as natural matrix CRM for organochlorine pesticides determination in fish and for marine environmental measurement purposes. The analytical methods used are described, and the obtained results were combined to calculate the mass fractions of the six detected organochlorine pesticides and their associated uncertainty values. It has been concluded that mass fractions of four pesticides are certified values. These are 1,1-(dichloroethylidene)bis[4-chlorobenzene](4,4′-DDE), 1,1-(2,2,-dichloroethylidene)bis[4-chlorobenzene] (4,4′-DDD), 1-chloro-2-[2,2,2-trichloro-1-(4-chlorophenyl)ethyl]benzene (2,4′-DDT) and 1,1-(2,2,2-trichloroethylidene)bis[4-chlorobenzene] (4,4′-DDT). Meanwhile, mass fractions of two pesticides were reference values. These are heptachlor and 1-chloro-2-[2,2-dichloro-1-(4-chlorophenyl)ethyl]benzene (2,4′-DDD).