Homologous Expression and Quantitative Analysis of T3SS-Dependent Secretion of TAP-Tagged XoAvrBs2 in Xanthomonas oryzae pv. oryzae Induced by Rice Leaf Extract

• Published in: Journal of microbiology and biotechnology Vol. 21; no. 7; pp. 679 - 685
• Main Authors: Kim, S.H., Konkuk University, Seoul, Republic of Korea, Lee, S.E., Monsanto Korea, Inc., Vegetable Seeds Division, Cheongwon, Republic of Korea, Hong, M.K., Konkuk University, Seoul, Republic of Korea, Song, N.H., Sangmyung University, Seoul, Republic of Korea, Yoon, B., Kyonggi University, Suwon, Republic of Korea, Viet, P.T., Konkuk University, Seoul, Republic of Korea, Ahn, Y.J., Kyonggi University, Suwon, Republic of Korea, Lee, B.M., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Jung, J.W., Konkuk University, Seoul, Republic of Korea, Kim, K.P., Konkuk University, Seoul, Republic of Korea, Han, Y.S., Konkuk University, Seoul, Republic of Korea, Kim, J.G., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kang, L.W., Konkuk University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.07.2011; 한국미생물·생명공학회
• Subjects: Bacterial Proteins - secretion; Biological and medical sciences; Biotechnology; Culture Media - chemistry; EFECTORES MOLECULARES; EFFECTEUR MOLECULAIRE; EFFECTORS; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Oryza - chemistry; Plant Extracts - metabolism; Protein Biosynthesis; Transcription, Genetic; Transcriptional Activation - drug effects; Xanthomonas - drug effects; Xanthomonas - genetics; Xanthomonas - metabolism; Xanthomonas oryzae pv. oryzae; 생물학; Pseudomonadales; oryzae; type III secretion system (TTSS); Monocotyledones; XoAvrBs2; Secretion; Xanthomonas oryzae; Xanthomonas oryzae pv; Extract; Plant leaf; effector; Type III secretion system; Oryza sativa; Gramineae; Angiospermae; Bacteria; Pseudomonadaceae; Spermatophyta; Quantitative analysis; Rice
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1102.02011

Xanthomonas oryzae pv. oryzae (Xoo) produces a putative effector, XoAvrBs2. We expressed XoAvrBs2 homologously in Xoo with a TAP-tag at the C-terminus to enable quantitative analysis of protein expression and secretion. Addition of rice leaf extracts from both Xoo-sensitive and Xoo-resistant rice cultivars to the Xoo cells induced expression of the XoAvrBs2 gene at the transcriptional and translational levels, and also stimulated a remarkable amount of XoAvrBs2 secretion into the medium. In a T3SS-defective Xoo mutant strain, secretion of the TAP-tagged XoAvrBs2 was blocked. Thus, we elucidated the transcriptional and translational expressions of the XoAvrBs2 gene in Xoo was induced in vitro by the interaction with rice and the induced secretion of XoAvrBs2 was T3SS-dependent. It is the first report to measure the homologous expression and secretion of XoAvrBs2 in vitro by rice leaf extract. Our system for the quantitative analysis of effector protein expression and secretion could be generally used for the study of host-pathogen interactions.