Transformation of Brassica napus with Agrobacterium tumefaciens based vectors

A reproducible system to produce transgenic Brassica napus plants has been developed using stem segments. Stem segments from 6-7 week old plants were inoculated with an Agrobacterium tumefaciens strain containing a disarmed tumor-inducing plasmid pTiT37-SE carrying a chimeric bacterial gene encoding...

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Bibliographic Details
Published inPlant cell reports Vol. 6; no. 5; pp. 321 - 325
Main Authors Fry, J, Barnason, A, Horsch, R.B
Format Journal Article
LanguageEnglish
Published Germany 01.10.1987
Subjects
Agrobacterium tumefaciens
Brassica napus
breeding methods
gene expression
genetic engineering
plant breeding
transformation
transgenic plants
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Summary:A reproducible system to produce transgenic Brassica napus plants has been developed using stem segments. Stem segments from 6-7 week old plants were inoculated with an Agrobacterium tumefaciens strain containing a disarmed tumor-inducing plasmid pTiT37-SE carrying a chimeric bacterial gene encoding kanamycin resistance (pMON200). Stem explants were cocultured for 2 days before transfer to kanamycin selection medium. Shoots regenerated directly from the explant in 3-6 weeks and were excised, dipped in Rootone®, and rooted in soil. Transformation was confirmed by opine production, kanamycin resistance, and DNA blot hybridization in the primary transformants. Final proof of transformation was demonstrated by the co-transfer of opine production and kanamycin resistance to progeny in a Mendelian fashion. Over 200 transgenic Brassica napus plants have been produced using this system.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00269550