Rapid high resolution imaging with a dual-channel scanning technique

A spatial shift between channels in a dual-beam raster-scan imaging system introduces a temporal separation between images from the two channels that can be much shorter than the frame rate of the system. The technique is demonstrated by measuring the velocity of erythrocytes in the retinal capillar...

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Bibliographic Details
Published inOptics letters Vol. 41; no. 8; p. 1881
Main Authors de Castro, Alberto, Huang, Gang, Sawides, Lucie, Luo, Ting, Burns, Stephen A
Format Journal Article
LanguageEnglish
Published United States 15.04.2016
Subjects
Blood Flow Velocity
Capillaries - cytology
Erythrocytes - cytology
Fovea Centralis - cytology
Humans
Optical Imaging - methods
Retinal Vessels - cytology
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Summary:A spatial shift between channels in a dual-beam raster-scan imaging system introduces a temporal separation between images from the two channels that can be much shorter than the frame rate of the system. The technique is demonstrated by measuring the velocity of erythrocytes in the retinal capillaries. We used an adaptive optics scanning laser ophthalmoscope and introduced a temporal separation between imaging channels of 4.7 ms. We imaged three subjects and measured changing capillary blood flow velocity at the pulse rate. Since the time shift between channels is easily and continuously adjustable, this method can be used to measure rapidly changing events in any raster scan system with little added complexity.
ISSN:1539-4794
DOI:10.1364/OL.41.001881