Synthesis of poly hydroxypropyl methacrylate cryogel incorporated with Zn/Ce substituted hydroxyapatite nanoparticles for rejuvenation of femoral fracture treatment in a rat model

• Published in: Journal of photochemistry and photobiology. B, Biology Vol. 201; p. 111651
• Main Authors: Zhou, Hongjie, Jiao, Huiping, Xu, Jingwei, Liu, Yulong, Wei, Shujun
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.12.2019; Elsevier BV
• Subjects: Antibacterial activity; Bioceramics; Biocompatibility; Biomaterials; Biomedical materials; Biomimetics; Bone; Cerium; Cryogel; Cytology; E coli; Functional groups; Hydroxyapatite; Hydroxypropyl methacrylate; Mechanical properties; Microorganisms; Mineralization; Morphology; Multiplication; Nanocomposites; Nanoparticles; Ossification; Osteoblast cell; Protein adsorption; Substitutes; Zinc; Osteoblast cell; Antibacterial activity; Biocompatibility; Hydroxyapatite; Bone; Cryogel
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2019.111651

Designing biomimetic biomaterials influenced by the common complex structure of hard tissues is yet a test these days. The control of bio-mineralization procedure onto biomaterials should be assessed before the use in medical applications. Coming to the bone rejuvenation applications, this work assessed the in vitro antibacterial activity and interacting between osteoblast cells (MG63) on poly (hydroxypropyl methacrylate) (PHPMA) cryogel consolidated with Zn/Ce substituted hydroxyapatite (MHAp) nanocomposite (PHPMA/MHAp). Osteoblast cell multiplication, morphology, and metabolic action were assessed through various conventions. The functional group, texture, mechanical properties, and protein adsorption profiles of the fabricated nanocomposite were analyzed by the FTIR, XRD, SEM, and mechanical examinations, respectively. The bacterial activity of nanocomposites was additionally assessed against E. coli and S. aureus microorganisms, individually. Nanocomposite advanced endo-chondral ossification at the messed up parts of the bone deformity than cryogel did. These results recommend that PHPMA/MHAp nanocomposites joined the good innate properties of each polymer and bioceramic, giving a mechanically powerful, cell-responsive, and permeable stage for hard tissue applications. [Display omitted] •PHPMA/MHAp nanocomposites specimens were successfully prepared by lyophilization molding.•The fabricated nanocomposite was showed excellent osteoconductivity and fitting ability.•It is demonstrated that nanocomposite enhances new bone formation in vivo.•PHPMA/MHAp can be introduced as a promising alternative to conventional grafting materials.