Effect of Phenazine Methosulfate on Lipogenesis

• Published in: The Journal of biological chemistry Vol. 245; no. 10; pp. 2546 - 2548
• Main Authors: Katz, J, Wals, P A
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 25.05.1970
• Subjects: Adipose Tissue - metabolism; Animals; Cytoplasm - metabolism; Epididymis; Fasting; Fatty Acids - biosynthesis; Female; Glucose - biosynthesis; Lactates - metabolism; Lactation; Lipids - biosynthesis; Male; Mammary Glands, Animal - metabolism; Metabolism - drug effects; Mitochondria - metabolism; NAD - biosynthesis; Phenazines - pharmacology; Pregnancy; Pyruvates - biosynthesis; Rats
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)63105-9

The effect of phenazine methosulfate (PMS) on lipogenesis of rat epididymal fat pad tissue was studied. PMS at concentrations of 10 -6 to 10 -5 m stimulates l -lactate oxidation and, most markedly, fatty acid synthesis, without affecting glycerol synthesis. Maximal stimulation of lipogenesis occurs at about 10 -5 m . With tissue of rats fed ad libitum , increase in fatty acid ranged from 300 to 1800%. There was no stimulation of synthesis from pyruvate or glucose. Similar effects of PMS on lipogenesis were also seen with mammary gland slices of lactating rats. It is concluded that there is limited capability for the transfer of cytoplasmic reducing equivalents into mitochondria. The effect of PMS is the result of oxidation of excess cytoplasmic reducing equivalents and regeneration of DPN.