Antioxidant Response and Calcium-Dependent Protein Kinases Involvement in Canola (Brassica napus L.) Tolerance to Drought

• Published in: Agronomy (Basel) Vol. 12; no. 1; p. 125
• Main Authors: Ahmadi, Hossein, Abbasi, Alireza, Taleei, Alireza, Mohammadi, Valiollah, Pueyo, José J.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.01.2022
• Subjects: Abiotic stress; Agricultural production; Antioxidants; Brassica; Brassica napus; Calcium; calcium-dependent protein kinases; Canola; Catalase; CDPK; Cultivars; Drought; Drought resistance; Electrolyte leakage; Electrolytes; Enzymes; Factorial experiments; Gene expression; Genes; Genetic transformation; Genotypes; Guaiacol; Heat; Irrigation; Kinases; Lipid peroxidation; Lipids; Loam soils; Metabolism; Moisture content; Molecular modelling; Nitrogen; Oilseeds; Peroxidase; Peroxidation; Phosphorylation; Photosystem II; Physiology; Plant breeding; Protein kinase; Proteins; Rape plants; rapeseed; Superoxide dismutase; Water content; Water deficit; Water stress
• ISSN: 2073-4395; 2073-4395
• DOI: 10.3390/agronomy12010125

Canola is an important temperate oil crop that can be severely affected by drought. Understanding the physiological and molecular mechanisms involved in canola tolerance to water deficit is essential to obtain drought-tolerant productive cultivars. To investigate the role of antioxidant response and the possible involvement of calcium-dependent protein kinases (CDPKs) in canola tolerance to drought, we analyzed four genotypes with different sensitivity to water stress. Leaf relative water content, canopy temperature, PSII efficiency, electrolyte leakage index and lipid peroxidation were used as indicators to classify the cultivars as drought-tolerant or drought-sensitive. Antioxidant enzymes superoxide dismutase, guaiacol peroxidase and catalase displayed significantly higher activities in drought-tolerant than in drought-sensitive cultivars subjected to water deficit, suggesting that the efficiency of the antioxidant response is essential in canola drought tolerance. The increased expression of genes BnaCDPK6 and BnaCDPK14 under drought conditions, their differential expression in drought-tolerant and drought-sensitive genotypes, and the presence of multiple cis-acting stress-related elements in their promoter regions suggest that CDPKs are part of the signaling pathways that regulate drought response in canola. We propose the BnaCDPK genes and their regulator elements as potential molecular targets to obtain drought-tolerant productive canola cultivars through breeding or genetic transformation.