Structures of two major histocompatibility complex class I genes of the rainbow trout (Oncorhynchus mykiss)

• Published in: Immunogenetics (New York) Vol. 54; no. 3; pp. 193 - 199
• Main Authors: Shum, Benny P, Mason, Patricia M, Magor, Katharine E, Flodin, Laura R, Stet, René J M, Parham, Peter
• Format: Journal Article
• Language: English
• Published: United States 01.06.2002
• Subjects: Amino Acid Sequence; Animals; Celbiologie en Immunologie; Cell Biology and Immunology; Fish Proteins - genetics; Genes, MHC Class I; Genetic Linkage; Histocompatibility Antigens Class I - genetics; Leerstoelgroep Celbiologie en immunologie; Molecular Sequence Data; Oncorhynchus mykiss - genetics; Phylogeny; Pseudogenes; Sequence Alignment; Sequence Analysis, DNA; WIAS
• ISSN: 0093-7711; 1432-1211
• DOI: 10.1007/s00251-002-0450-z

Here we describe two rainbow trout major histocompatibility complex (MHC) class I genes characterized from lambda phage genomic clones prepared from a single fish. Clone GC71 contains all exons except a leader peptide-encoding exon. An open reading frame is maintained, and thus the gene MhcOnmy-U71 could be expressed in this individual. The class I gene found on clone GC41 lacks exons encoding the leader peptide and cytoplasmic domain. This gene, MhcOnmy-U41p, is a pseudogene due to a deletion in the alpha(2) domain-encoding exon causing premature termination. Both the Onmy-U71 and Onmy-U41p genes are distinguished by long introns between the exons encoding the alpha(1) and alpha(2) domains. Clone GC41 also contains the 3' exons of the LMP7/ PSMB8 gene encoding the gamma-interferon-induced proteosome subunit of rainbow trout.