Fast and simple liquid chromatographic determination of nonphosphorylated thiamine in infant formula, milk and other foods

A very fast and simple method for determination of nonphosphorylated thiamine in infant formula products, milk, and other nonfortified foods using reverse-phase ion-pairing liquid chromatography (LC) has been developed. Sample preparation consists of merely acid treatment to precipitate protein, fol...

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Bibliographic Details
Published inJournal of the Association of Official Analytical Chemists Vol. 73; no. 5; p. 792
Main Authors Nicolas, E.C. (Ganes Chemicals, Inc., Pennsville, NJ), Pfender, K.A
Format Journal Article
LanguageEnglish
Published United States 01.09.1990
Subjects
ALIMENT POUR NOURRISSON
ALIMENTOS
ALIMENTOS PARA NINOS
Animals
CHEMICAL COMPOSITION
CHROMATOGRAPHIE
CHROMATOGRAPHY
Chromatography, Liquid
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CROMATOGRAFIA
Dairy Products - analysis
DIET
DIETA
Eggs - analysis
Food Analysis
FOODS
Humans
Indicators and Reagents
Infant
Infant Food - analysis
INFANT FOODS
LAIT
LECHE
MILK
Milk - chemistry
PRODUIT ALIMENTAIRE
REGIME ALIMENTAIRE
Solutions
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
THIAMIN
THIAMINE
Thiamine - analysis
TIAMINA
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Summary:A very fast and simple method for determination of nonphosphorylated thiamine in infant formula products, milk, and other nonfortified foods using reverse-phase ion-pairing liquid chromatography (LC) has been developed. Sample preparation consists of merely acid treatment to precipitate protein, followed by gravity filtration. No concentration, extraction, derivatization, or preliminary column cleanup is necessary. The chromatography is done on muBondapack C18 with an aqueous mobile phase containing 0.15% sodium hexane sulfonate, 20% MeOH, 1.5% HOAc, and 0.1% EDTA at a flow rate of 2.5 mL/min. Ultraviolet detection at 248 nm is used. A typical run takes 7 min, and 60 samples can be processed in 4 h. Results average from 96 to 104% of theory for the infant formula products analyzed. A 99 to 103% recovery of spike has been demonstrated. Method precision is good (2 to 4% RSD, short-term, and 2 to 5% RSD, long-term, depending on sample type). Peak separation from thiamine phosphate esters is achieved. Specificity is demonstrated by UV spectral scan and absorbance ratios. Equivalency to a microbial method (validated against the official AOAC fluorometric method) was established. The method is used for high-volume quality control testing of milk-based infant formula products in the ready-to-use, concentrate, or powder form.
Bibliography:Q04
9120017
ISSN:0004-5756
DOI:10.1093/jaoac/73.5.792