The detection of glycoproteins immunologically related to RSV gp85 in uninfected avian cells and in sera from uninfected birds

• Published in: Virology (New York, N.Y.) Vol. 86; no. 1; pp. 226 - 240
• Main Authors: Bosch, Valerie, Kurth, Reinhard, Smart, John E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.1978
• Subjects: Animals; Antigens, Viral; Avian Leukosis Virus - immunology; Avian Myeloblastosis Virus - immunology; Avian Sarcoma Viruses - immunology; Cells, Cultured; Chickens - immunology; Cross Reactions; Epitopes; Glycoproteins - blood; Glycoproteins - immunology; Rous sarcoma virus; Viral Proteins - immunology
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/0042-6822(78)90023-5

The experiments described in this study deal with the detection of components immunologically related to oncornaviral glycoprotein (gp85) in uninfected cell extracts and sera. Cell extracts from chick helper factor- (chf) negative fibroblasts contain components detected by two of the three antisera used. Chf(+) chicken cell extracts contain in addition an antigenic determinant, presumably viral gp85, recognized by the third antiserum. The sera from uninfected chickens may contain two components immunologically related to gp85: One component recognized in a radioimmuno-precipitation assay (RIA) employing anti-AMV gp85 serum is present in high amounts in the sera of chf(+) and chf(-) birds, while a second component detected in a RIA employing anti-Prague B RSV serum is present only in the sera of chf(+) birds. Some biochemical studies dealing with the properties and size of the cross-reacting components are described. It is postulated that the components which immunologically cross-react with, but are not identical to, endogenous viral glycoprotein are products of genes related to the env gene of RAV-0 which, however, are nonfunctional as viral glycoprotein.