Identification of an insertion sequence, IS1203 variant, in a Shiga toxin 2 gene of Escherichia coli O157:H7

An insertion sequence composed of 1310 bp was found in Shiga toxin 2 genes of some isolates of Escherichia coli O157:H7. This insertion sequence showed extremely high homology with IS1203 of E. coli O111:H(-). This IS1203 variant was inserted in the region encoding the amino-terminus of the B subuni...

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Bibliographic Details
Published inJournal of bioscience and bioengineering Vol. 87; no. 1; pp. 93 - 96
Main Authors KUSUMOTO, M, NISHIYA, Y, KAWAMURA, Y, SHINAGAWA, K
Format Journal Article
LanguageEnglish
Published Amsterdarm Elsevier Science 1999
Subjects
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Genetics
Microbiology
Toxin
Gene
Nucleotide sequence
Escherichia coli
Pathogenic
Bacteria
Homology
Enterobacteriaceae
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Summary:An insertion sequence composed of 1310 bp was found in Shiga toxin 2 genes of some isolates of Escherichia coli O157:H7. This insertion sequence showed extremely high homology with IS1203 of E. coli O111:H(-). This IS1203 variant was inserted in the region encoding the amino-terminus of the B subunit with a duplication of 3 bp at the target site, resulting in inactivation of the Shiga toxin 2 gene.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1389-1723
1347-4421
DOI:10.1016/s1389-1723(99)80014-0