Tailoring magnetic PLGA nanoparticles suitable for doxorubicin delivery

• Published in: Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology Vol. 16; no. 1; pp. 1 - 13
• Main Authors: Tansık, Gülistan, Yakar, Arzu, Gündüz, Ufuk
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 2014; Springer; Springer Nature B.V
• Subjects: Biocompatibility; Biological and medical sciences; Biotechnology; Characterization and Evaluation of Materials; Chemistry and Materials Science; Condensed matter: electronic structure, electrical, magnetic, and optical properties; Condensed matter: structure, mechanical and thermal properties; Cross-disciplinary physics: materials science; rheology; Cytotoxicity; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Inorganic Chemistry; Iron oxides; Lasers; Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties; Magnetic fields; Magnetic properties and materials; Materials Science; Methods of nanofabrication; Methods. Procedures. Technologies; Nanoparticles; Nanotechnology; Optical Devices; Optics; Others; Photonics; Physical Chemistry; Physics; Polymers; Research Paper; Side effects; Small particles and nanoscale materials; Studies of specific magnetic materials; Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties); Various methods and equipments; Targeted anti-cancer drug delivery; PLGA; MCF-7; Doxorubicin; Nanomedicine; Fe; O; Encapsulation; Particle size; Drug delivery systems; Iron oxide; Toxicity; Cytotoxicity; Drug carrier; Superparamagnetism; Selectivity; Magnetic nanomaterial; Nanostructures; Coatings; Nanoparticles; Nanometer scale; Oleic acid; Targeted drug; Biocompatibility; Magnetic materials; Polymers; Medical application; Nanomaterial synthesis; External fields; Antineoplastic drugs; Cell killing; Tumor cells; Magnetic field effects; Magnetic particles; Quantity ratio; Tumours
• ISSN: 1388-0764; 1572-896X
• DOI: 10.1007/s11051-013-2171-7

One of the main problems of current cancer chemotherapy is the lack of selectivity of anti-cancer drugs to tumor cells, which leads to systemic toxicity and adverse side effects. In order to overcome these limitations, researches on controlled drug delivery systems have gained much attention. Nanoscale-based drug delivery systems provide tumor targeting. Among many types of nanocarriers, superparamagnetic nanoparticles with their biocompatible polymer coatings can be targeted to an intented site by an external magnetic field. Thus, the drug can be carried to the targeted site safely. The aim of this study is to prepare poly( dl -lactic- co -glycolic acid) (PLGA)-coated magnetic nanoparticles and load anti-cancer drug, doxorubicin to them. For this purpose, magnetite (Fe 3 O 4 ) iron oxide nanoparticles were synthesized as a magnetic core material (MNP) and then coated with oleic acid. Oleic acid-coated MNP (OA-MNP) was encapsulated into PLGA. Effects of different OA-MNP/PLGA ratios on magnetite entrapment efficiency were investigated. Doxorubicin-loaded magnetic polymeric nanoparticles (DOX-PLGA-MNP) were prepared. After the characterization of prepared nanoparticles, their cytotoxic effects on MCF-7 cell line were studied. PLGA-coated magnetic nanoparticles (PLGA-MNP) had a proper size and superparamagnetic character. The highest magnetite entrapment efficiency of PLGA-MNP was estimated as 63 % at 1:8 ratio. Cytotoxicity studies of PLGA-MNP did not indicate any notable cell death between the concentration ranges of 2 and 125 μg/ml. Drug loading efficiency was estimated as 32 %, and it was observed that DOX-PLGA-MNP showed significant cytotoxicity on MCF-7 cells compared to PLGA-MNP. The results showed that prepared nanoparticles have desired size and superparamagnetic characteristics without serious toxic effects on cells. These nanoparticles may be suitable for targeted drug delivery applications.