Acetylhomoserine and Methionine Biosynthesis in Neurospora

This communication reports several independent observations which suggest that O -acetylhomoserine is an intermediate in methionine biosynthesis in Neurospora . Indirect evidence has long implicated three unlinked genes ( me-3, me-5, me-7 ) in a hypothetical series of reactions leading to the format...

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Published inThe Journal of biological chemistry Vol. 241; no. 16; pp. 3861 - 3863
Main Authors Nagai, S, Flavin, M
Format Journal Article
LanguageEnglish
Published United States American Society for Biochemistry and Molecular Biology 25.08.1966
Subjects
Acetates - biosynthesis
Carbon Isotopes - analysis
Chromatography, Paper
Electrophoresis
Methionine - biosynthesis
Neurospora - metabolism
Serine - biosynthesis
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Summary:This communication reports several independent observations which suggest that O -acetylhomoserine is an intermediate in methionine biosynthesis in Neurospora . Indirect evidence has long implicated three unlinked genes ( me-3, me-5, me-7 ) in a hypothetical series of reactions leading to the formation of cystathionine from homoserine and cysteine in Neurospora , but the metabolic defects associated with mutations in these genes have not been ascertained. It has now been shown that me-5 mutants, but not me-3 or me-7 , will grow in the presence of O -acetylhomoserine. Second, when grown in the presence of methionine and labeled homoserine, me-5 mutants fail to accumulate two compounds which are formed by me-3, me-7 , and wild type: O -acetylhomoserine, and a larger amount of 4- O -acetyl-2,4-dihydroxybutyrate. Third, me-5 mutants lack an enzyme catalyzing an exchange between labeled homoserine and O -acetylhomoserine.
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ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)99850-9