Isolation of a Novel Freshwater Agarolytic Cellvibrio sp. KY-YJ-3 and Characterization of Its Extracellular β-Agarase

• Published in: Journal of microbiology and biotechnology Vol. 20; no. 10; pp. 1378 - 1385
• Main Authors: Rhee, Y.J., Kyungpook National University, Daegu, Republic of Korea, Han, C.R., Kyungpook National University, Daegu, Republic of Korea, Kim, W.C., Kyungpook National University, Daegu, Republic of Korea, Jun, D.Y., Kyungpook National University, Daegu, Republic of Korea, Rhee, I.K., Kyungpook National University, Daegu, Republic of Korea, Kim, Y.H., Kyungpook National University, Daegu, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.10.2010; 한국미생물·생명공학회
• Subjects: agarolytic Cellvibrio sp; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; beta-agarase; Biological and medical sciences; Biotechnology; Cellvibrio - classification; Cellvibrio - enzymology; Cellvibrio - genetics; Cellvibrio - isolation & purification; Enzyme Stability; Fresh Water - microbiology; Freshwater environment; Fundamental and applied biological sciences. Psychology; Glycoside Hydrolases - chemistry; Glycoside Hydrolases - genetics; Glycoside Hydrolases - metabolism; Kinetics; Molecular Sequence Data; Molecular Weight; neoagarooligosaccharide; Phylogeny; PURIFICACION; PURIFICATION; 생물학; agarolytic Cellvibrio sp; neoagarooligosaccharide; Purification; β-agarase; Enzyme; Fresh water; Extracellular; Glycosylases; Characterization; Freshwater environment; Glycosidases; Agarase; Hydrolases; Environment; Isolation

A novel agarolytic bacterium, KY-YJ-3, producing extracellular agarase, was isolated from the freshwater sediment of the Sincheon River in Daegu, Korea. On the basis of Gram-staining data, morphology, and phylogenetic analysis of the 16S rDNA sequence, the isolate was identified as Cellvibrio sp. By...