Effect of light on the organogenic ability of garlic roots using a one-step in vitro system

A simple and efficient garlic in vitro shoot regeneration protocol has been developed. This system uses axenic root tips cultivated from the beginning in the presence of light and does not require any change or refreshing of the original medium during the entire process. The application of light fro...

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Bibliographic Details
Published inPlant cell reports Vol. 22; no. 10; pp. 721 - 724
Main Authors Martin-Urdiroz, N, Garrido-Gala, J, Martin, J, Barandiaran, X
Format Journal Article
LanguageEnglish
Published Germany Springer Nature B.V 01.05.2004
Subjects
Allium sativum
callus
culture media
Culture Techniques - methods
garlic
Garlic - growth & development
genotype
in vitro regeneration
Invertebrates
Light
lighting
micropropagation
Organogenesis
phenotypic variation
plant morphology
Plant Roots - growth & development
Plant Shoots - growth & development
Regeneration
root tips
tissue culture
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Summary:A simple and efficient garlic in vitro shoot regeneration protocol has been developed. This system uses axenic root tips cultivated from the beginning in the presence of light and does not require any change or refreshing of the original medium during the entire process. The application of light from the beginning of the culture process did not affect the callus formation rate but did significantly improve the explant regeneration ability. In a 2-month period it was possible to obtain up to 250 shoots per gram of callus.
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ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-003-0744-0