Stacking phenomena in electromigration: From basic principles to practical procedures

The review describes principles of procedures and techniques used in capillary zone electrophoresis for enhancement of sensitivity that are based on increasing analyte mass in its zone during the electromigration process, for which the term stacking is generally used. Attention is paid to intrinsic...

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Bibliographic Details
Published inElectrophoresis Vol. 24; no. 3; pp. 466 - 485
Main Authors Urbánek, Marek, Křivánková, Ludmila, Boček, Petr
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 01.02.2003
WILEY‐VCH Verlag
Subjects
Capillary zone electrophoresis
Chromatography, Micellar Electrokinetic Capillary - methods
Electrophoresis, Capillary - methods
Isotachophoresis
Micelles
Review
Sensitivity enhancement
Stacking
Static Electricity
Sweeping
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Summary:The review describes principles of procedures and techniques used in capillary zone electrophoresis for enhancement of sensitivity that are based on increasing analyte mass in its zone during the electromigration process, for which the term stacking is generally used. Attention is paid to intrinsic stacking in samples with low conductivity, transient isotachophoretic stacking applied in samples with high conductivity, and sweeping in micellar electrokinetic chromatography. Principles of these stacking schemes are explained, new procedures and instrumental arrangements are discussed, and all contributions involving stacking principles that have been published since the year 2000 are surveyed.
Bibliography:ark:/67375/WNG-TKL86KT2-L
ArticleID:ELPS200390056
istex:67C91F14F65C742FA22E55B0B6B6A80919B7B273
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200390056