Single-cell gel electrophoresis (SCG)-A review and discussion

Single-cell gel electrophoresis (SCG) is a simple, sensitive and effective technique. Being able to reflect quantitatively the genotoxicity of many hazardous agents, it is promising for application in environmental genotoxic monitoring and the study of carcinogenesis. In clinics, it can be used to e...

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Published inEnvironmental health and preventive medicine Vol. 2; no. 2; pp. 53 - 58
Main Authors Lu, Y, Takeshita, T, Morimoto, K
Format Journal Article
LanguageEnglish
Published England BioMed Central 01.07.1997
Springer-Verlag
Subjects
Bioindicators
Biomarkers
Cancer
Cancer therapies
Carcinogenesis
Chromosome aberrations
DNA
DNA repair
Electrophoresis
Environmental health
Genotoxicity
Review
Reviews
Sister chromatid exchange
Single-cell gel electrophoresis
Biomarker
Comet assay
DNA strand breaks
Genotoxicity
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Summary:Single-cell gel electrophoresis (SCG) is a simple, sensitive and effective technique. Being able to reflect quantitatively the genotoxicity of many hazardous agents, it is promising for application in environmental genotoxic monitoring and the study of carcinogenesis. In clinics, it can be used to evaluate the DNA repair ability and monitor DNA breaks during cancer therapy. As a biomarker, it has its own merits and limitations, being different from other biomarkers such as sister chromatid exchange (SCE) test and micronuclei (MN) assay. In many studies, it is more sensitive than SCE or MN. Combination studies with other biomarkers like SCE, MN, chromosomal aberration, bcl-2 and genetic polymorphisms have begun to demonstrate its great importance for the understanding of carcinogenesis and the genotoxicities of environmental factors.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:1342-078X
1347-4715
DOI:10.1007/BF02931964