Synthesis and characterization of four novel palladium(II) and platinum(II) complexes with 1‐(2‐aminoethyl)pyrrolidine, diclofenac and mefenamic acid: In vitro effect of these complexes on human serum paraoxanase1 activity

• Published in: Journal of biochemical and molecular toxicology Vol. 32; no. 4; pp. e22043 - n/a
• Main Authors: Dilek, Esra, Caglar, Sema, Erdogan, Kübra, Caglar, Bülent, Sahin, Onur
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.04.2018
• Subjects: Crystal structure; Crystallography; Diclofenac; enzyme inhibition; Enzymes; High temperature; Inflammation; Mefenamic acid; Nonsteroidal anti-inflammatory drugs; Palladium; palladium(II) and platinum(II) complexes; paraoxonase1; Platinum; Pyrrolidine; paraoxonase1; crystal structure; enzyme inhibition; palladium(II) and platinum(II) complexes
• ISSN: 1095-6670; 1099-0461
• DOI: 10.1002/jbt.22043

In this study, the effects of four novel mononuclear palladium(II) and platinum(II) complexes on the activity of human serum paraoxanase1 were examined. First, four novel mononuclear palladium(II) and platinum(II) complexes were synthesized with a nitrogen donor ligand 1‐(2‐aminoethyl)pyrrolidine and nonsteroidal anti‐inflammatory drugs diclofenac, mefenamic acid. These complexes were characterized by spectroscopic, thermal, and elemental analyses. The crystal structures of complex [Pd(2‐amepyr)2](dicl)2 1 and [Pd(2‐amepyr)2](mef)2 3 were determined by X‐ray crystallography. Then, paraoxonase1 enzyme was purified from human serum. The effects of these complexes on enzyme were evaluated in vitro. The complexes consist of the cationic unit and the counterions. The diclofenac and mefenamic acid acted as a counterion in the complexes. It was observed that all the complexes were stable up to high temperatures. These complexes, even at low doses, inhibited the activity of the enzyme with different inhibition mechanisms.