Digital light processing printed hydrogel scaffolds with adjustable modulus

• Published in: Scientific reports Vol. 14; no. 1; pp. 15695 - 11
• Main Authors: Xu, Feng, Jin, Hang, Wu, Huiquan, Jiang, Acan, Qiu, Bin, Liu, Lingling, Gao, Qiang, Lin, Bin, Kong, Weiwei, Chen, Songyue, Sun, Daoheng
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.07.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/61/2035; 639/166/985; 639/301/923; Acrylic Resins - chemistry; Adjustable modulus; Alginates - chemistry; Alginic acid; Biocompatible Materials - chemistry; Biomaterials; Digital light processing; Double network hydrogels; Elastic Modulus; Fabrication; Humanities and Social Sciences; Humans; Hydrogel scaffolds; Hydrogels; Hydrogels - chemistry; Iron; Light; multidisciplinary; Polyacrylamide; Printing, Three-Dimensional; Science; Science (multidisciplinary); Tissue Engineering - methods; Tissue Scaffolds - chemistry; Tissues; Adjustable modulus; Hydrogel scaffolds; Digital light processing; Double network hydrogels
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-66507-x

Hydrogels are extensively explored as biomaterials for tissue scaffolds, and their controlled fabrication has been the subject of wide investigation. However, the tedious mechanical property adjusting process through formula control hindered their application for diverse tissue scaffolds. To overcome this limitation, we proposed a two-step process to realize simple adjustment of mechanical modulus over a broad range, by combining digital light processing (DLP) and post-processing steps. UV-curable hydrogels (polyacrylamide-alginate) are 3D printed via DLP, with the ability to create complex 3D patterns. Subsequent post-processing with Fe 3+ ions bath induces secondary crosslinking of hydrogel scaffolds, tuning the modulus as required through soaking in solutions with different Fe 3+ concentrations. This innovative two-step process offers high-precision (10 μm) and broad modulus adjusting capability (15.8–345 kPa), covering a broad range of tissues in the human body. As a practical demonstration, hydrogel scaffolds with tissue-mimicking patterns were printed for cultivating cardiac tissue and vascular scaffolds, which can effectively support tissue growth and induce tissue morphologies.