Variability and relationships of the Far Eastern species of sculpins Myoxocephalus and Megalocottus (Cottidae) based on mtDNA markers and karyological data

• Published in: Russian journal of genetics Vol. 50; no. 9; pp. 949 - 956
• Main Authors: Podlesnykh, A. V, Moreva, I. N
• Format: Journal Article
• Language: English
• Published: Moscow Springer-Verlag 01.09.2014; Pleiades Publishing
• Subjects: Animal Genetics; Animal Genetics and Genomics; Biomedical and Life Sciences; Biomedicine; cell nucleolus; chromosomes; cytochrome b; cytochrome-c oxidase; genes; haplotypes; Human Genetics; interspecific variation; karyotyping; Megalocottus; Microbial Genetics and Genomics; mitochondrial DNA; monophyly; Myoxocephalus stelleri; Okhotsk Sea; Sea of Japan; Japan; Cytb Gene; Karyological Data; Active Nucleolus Organizer; Cytb Sequence; Eastern Species
• ISSN: 1022-7954; 1608-3369
• DOI: 10.1134/s1022795414090117

The phylogenetic relationships among five species of sculpins, including Myoxocephalus stelleri, M. brantii, M. jaok, M. ochotensis, and Megalocottus platycephalus, were estimated from the sequence variability of the mtDNA cytochrome b (cytb) and cytochrome oxidase 1 (CO1) genes. Analysis of the topologies of combined phylogenetic trees showed that all of the morphologically described species from different genera represented monophyletic groups with high support of branch robustness. Haplotypes with different karyotypes from different geographical localities with an intragroup nucleotide diversity of 0.42% were combined into the M. stelleri clade. All of the species (except for M. stelleri from the Sea of Japan and the Okhotsk Sea coast of the Hokkaido Island) were characterized by relatively low values of the intragroup variation, along with high values of interspecific variation of mtDNA-encoded markers. Studies of the M. stelleri karyotypes using Ag-banding showed that karyotypes of the individuals from the Sea of Japan and Okhotsk differed not only in the number of chromosomes (2n) but also in the number of active nucleolus organizers (NO) and stained NO blocks, calling into question whether they belong to a single species. The observed discrepancy between the phylogenetic topologies and karyological data is discussed in relation to the informative capacity of mtDNA fragments and the phenomenon of intraspecific chromosomal polymorphism.