Effects of differentiated membranes on the developmental program of the cellular slime mold

• Published in: Developmental biology Vol. 51; no. 1; pp. 77 - 85
• Main Authors: Tuchman, Jessica, Smart, John E., Lodish, Harvey F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.1976
• Subjects: Acetylglucosaminidase - metabolism; Alkaline Phosphatase - metabolism; Cell Membrane; Dictyostelium - enzymology; Dictyostelium - growth & development; Dictyostelium - ultrastructure; Dictyostelium discoideum; Enzyme Induction; Glucosidases - metabolism; Hydro-Lyases - metabolism; Hydrolases - metabolism; Isoenzymes - metabolism; Myxomycetes - enzymology; Threonine Dehydratase - metabolism; Transferases - metabolism; Tyrosine Transaminase - metabolism; UTP-Glucose-1-Phosphate Uridylyltransferase - metabolism
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1016/0012-1606(76)90123-8

In the preceding paper isolated aggregation phase membranes (prepared from Dictyostelium discoideum cells which had proceeded through 12–14 hr of the developmental cycle) were found to be capable of preventing the aggregation and subsequent morphological development of vegetative cells when mixed with these and plated under normal conditions for slime mold development. In this paper we have extended the investigations on the nature of this interaction by monitoring the display of several developmentally controlled enzymes. It appears that exogenously applied aggregation phase membrane preparations are capable of influencing biochemical events inside D. discoideum cells through their interaction with the cell surface. This interaction leads to the induction or accumulation of some developmentally controlled enzymes, as well as the repression or excretion of others. The results suggest that the formation and maintenance of correct cell-cell contacts during normal development may be of crucial importance. They also show that changes in the specific activity of some developmentally controlled enzymes may in certain conditions be wholly divorced from both morphogenesis and the normal sequence of induction.