Complete sequences and phylogenetic analyses of a Singapore isolate of broad bean wilt fabavirus

• Published in: Archives of virology Vol. 146; no. 1; pp. 135 - 147
• Main Authors: KOH, L. H, COOPER, J. I, WONG, S. M
• Format: Journal Article
• Language: English
• Published: Wien Springer 01.01.2001; New York, NY Springer Nature B.V
• Subjects: Bacteriology; Base Sequence; Biological and medical sciences; Broad bean wilt fabavirus; Capsid - genetics; Cloning, Molecular; Comoviridae; Endopeptidases - genetics; Epidemiology; Fabaceae - virology; Fabavirus - classification; Fabavirus - genetics; Fundamental and applied biological sciences. Psychology; Genome, Viral; Megakepasm erythrochlamys; Microbiology; Molecular Sequence Data; movement protein; Mycology; Pathogenicity, host-agent relations, miscellaneous strains, epidemiology; Phylogeny; Plant Viral Movement Proteins; Plants, Medicinal; RNA Helicases - genetics; RNA Replicase - genetics; Sequence Homology, Amino Acid; Singapore; Viral Proteins - genetics; VPg protein; Asia; Singapore; Fabavirus; Serine endopeptidases; Omptin; RNA; Nucleotide sequence; Enzyme; Transferases; Phylogeny; Comoviridae; DNA-directed RNA polymerase; Protein; Bean; Virus; Peptidases; Nucleotidyltransferases; Nucleotide; Hydrolases; Isolate; Genome
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s007050170197

The complete nucleotide (nt) sequence of a Singapore isolate of broad bean wilt fabavirus from Megakepasma erythrochlamys L., designated BBWV-ME, was determined. Its bipartite genome consisted of two positive-sense single-stranded ribonucleic acids (RNA). RNA1 (5951 nt in length) encoded a putative protease cofactor, nucleotide triphosphate (NTP)-binding domain (helicase), viral genome-linked protein (VPg), protease and RNA-dependent RNA polymerase (RdRp). RNA2 (3607 nt in length) encoded a putative movement protein (MP) and coat proteins (CP). Genome organization of BBWV-ME was similar to other viruses in the Comoviridae family. Phylogenetic analyses showed that fabaviruses were more closely related to the comoviruses than the nepoviruses.