Phylogenetic analysis of Histoplasma capsulatum var duboisii in baboons from archived formalin-fixed, paraffin embedded tissues

Histoplasma capsulatum var. duboisii (Hcd) infections have been well documented to cause chronic granulomatous disease, mainly involving the skin of baboons and humans in African countries primarily. This retrospective study classified the subspecies of Histoplasma and developed a phylogenetic tree...

Full description

Saved in:
Bibliographic Details
Published inMedical mycology (Oxford) Vol. 57; no. 2; p. 256
Main Authors Hensel, M, Hoffmann, A Rodrigues, Gonzales, M, Owston, M A, Dick, Jr, E J
Format Journal Article
LanguageEnglish
Published England 01.02.2019
Subjects
Africa - epidemiology
Animals
DNA, Fungal - genetics
DNA, Ribosomal Spacer - genetics
Formaldehyde
Genes, Fungal - genetics
Histoplasma - classification
Histoplasma - genetics
Histoplasma - isolation & purification
Histoplasmosis - epidemiology
Histoplasmosis - microbiology
Histoplasmosis - veterinary
Molecular Epidemiology
Papio - microbiology
Paraffin Embedding - veterinary
Phylogeny
Primate Diseases - epidemiology
Primate Diseases - microbiology
Retrospective Studies
Sequence Analysis, DNA
Texas - epidemiology
Texas
Africa
Online AccessGet more information

Cover

More Information
Summary:Histoplasma capsulatum var. duboisii (Hcd) infections have been well documented to cause chronic granulomatous disease, mainly involving the skin of baboons and humans in African countries primarily. This retrospective study classified the subspecies of Histoplasma and developed a phylogenetic tree utilizing DNA sequences extracted from formalin-fixed, paraffin embedded (FFPE) tissues from 9 baboons from a research colony in Texas histologically diagnosed with Hcd. Based on sequence analysis of ITS-2, Tub-1, and ARF, Hcd isolated from the archived samples closely aligns with the African clade and has 88% sequence homology with a sample isolated from an individual in Senegal.
ISSN:1460-2709
DOI:10.1093/mmy/myy003