Carnosic acid in green callus and regenerated shoots of Rosmarinus officinalis

Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarinus officinalis were grown on a basal nutrient medium for 9 weeks. The regenerants were shown by means of HPLC and mass spectrometry to contain carnosic acid, a diterpenoid with antioxidant and medicinal properties. Five-week-...

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Published inPlant cell reports Vol. 19; no. 5; pp. 500 - 503
Main Authors CARUSO, J. L, CALLAHAN, J, DECHANT, C, JAYASIMHULU, K, WINGET, G. D
Format Journal Article
LanguageEnglish
Published Berlin Springer 01.04.2000
Springer Nature B.V
Subjects
Biological and medical sciences
Biotechnology
carnosic acid
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
In vitro propagation: entire plant regeneration from tissues and cell cultures
Liquid chromatography
Mass spectrometry
Methods. Procedures. Technologies
Plant cells and fungal cells
Callus
Vegetals
Essential oil plant
Tissue culture
Antioxidant
Regeneration
Labiatae
Dicotyledones
Angiospermae
Rosmarinus officinalis
Diterpene
Spermatophyta
Shoot
Labiatae (Lamiaceae)
Key words Rosmarinus officinalis
Carnosic acid
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Summary:Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarinus officinalis were grown on a basal nutrient medium for 9 weeks. The regenerants were shown by means of HPLC and mass spectrometry to contain carnosic acid, a diterpenoid with antioxidant and medicinal properties. Five-week-old nodular green callus also contained carnosic acid, whereas non-green, undifferentiated callus maintained in the dark did not.
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ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050763