Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system

Tripartite efflux pumps and the type I secretion system of Gram-negative bacteria are large protein complexes that span the entire cell envelope. These complexes expel antibiotics and other toxic substances or transport protein toxins from bacterial cells. Elucidating the binary and ternary complex...

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Published inThe journal of microbiology Vol. 57; no. 3; pp. 185 - 194
Main Authors Jo, Inseong, Kim, Jin-Sik, Xu, Yongbin, Hyun, Jaekyung, Lee, Kangseok, Ha, Nam-Chul
Format Journal Article
LanguageEnglish
Published Seoul The Microbiological Society of Korea 01.03.2019
한국미생물학회
Subjects
Biomedical and Life Sciences
Life Sciences
Microbiology
Review
생물학
multidrug efflux pump
multidrug resistance
structure
cryo-electron microscopy
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Summary:Tripartite efflux pumps and the type I secretion system of Gram-negative bacteria are large protein complexes that span the entire cell envelope. These complexes expel antibiotics and other toxic substances or transport protein toxins from bacterial cells. Elucidating the binary and ternary complex structures at an atomic resolution are crucial to understanding the assembly and working mechanism. Recent advances in cryoelectron microscopy along with the construction of chimeric proteins drastically shifted the assembly models. In this review, we describe the current assembly models from a historical perspective and emphasize the common assembly mechanism for the assembly of diverse tripartite pumps and type I secretion systems.
ISSN:1225-8873
1976-3794
DOI:10.1007/s12275-019-8520-1