Transduction of inositol-fermenting ability demonstrating phylogenetic relationships among strains of Salmonella typhimurium

• Published in: Genetical Research Vol. 35; no. 2; pp. 215 - 224
• Main Authors: Old, D. C., Dawes, P. F. H., Barker, Ruth M.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.04.1980
• Subjects: Bacteriophages; Genetic Markers; Inositol - metabolism; Phylogeny; Salmonella typhimurium - classification; Salmonella typhimurium - genetics; Transduction, Genetic
• ISSN: 0016-6723; 1469-5073
• DOI: 10.1017/S0016672300014063

The production of Inl+ recombinants was readily demonstrated in transductional crosses from Inl+ donor strains to Inl− recipient strains from different biotypes and biogroups. None of numerous crosses between different pairs of strains from biotypes 25 and 26 and the biogroup FIRN (biotypes 29, 30, 31 and 32) gave Inl+ recombinants suggesting that the inl mutation was present at the same intragenic site in all of these strains. Strains of the FIRN biogroup (Fim−Inl−Rha−Bxyl−) are thought to have descended by successive rha and fim mutations from an ancestral bacterium of biotype 25 (Fim+Inl−Rha+Bxyl−). The sites of the inl mutations in other Inl− biotypes (9, 10, 27 and 25hi) were independent and each was different from that in strains from biotypes 25, 26 and FIRN.