The cytokine response of circulating peripheral blood mononuclear cells is changed after intravenous injection of lipopolysaccharide in cattle

• Published in: The veterinary journal (1997) Vol. 174; no. 1; pp. 170 - 175
• Main Authors: Jacobsen, S., Andersen, P.H., Aasted, B.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2007
• Subjects: animal models; Animals; anti-inflammatory activity; Bovine; Cattle - blood; Cattle - immunology; cattle diseases; Cytokine; cytokines; Cytokines - biosynthesis; Cytokines - blood; dairy cows; Female; Flow Cytometry - veterinary; immune response; immune system; immunosuppression (physiological); infectious diseases; inflammation; Inflammatory-stimulus-induced anergy; interferons; interleukins; Interleukins - blood; Interleukins - immunology; Intracellular staining; intravenous injection; leukocytes; Leukocytes, Mononuclear - immunology; Lipopolysaccharide; lipopolysaccharides; Lipopolysaccharides - pharmacology; long term effects; lymphocytes; monocytes; temporal variation; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - immunology; Lipopolysaccharide; Intracellular staining; Bovine; Cytokine; Inflammatory-stimulus-induced anergy
• DOI: 10.1016/j.tvjl.2006.06.006
• ISSN: 1090-0233

The aim of the study was to investigate lipopolysaccharide (LPS)-induced short and long term changes in capacity for intracellular cytokine-production of bovine circulating peripheral blood mononuclear cells (PBMCs). Eight dairy cows each received three intravenous injections of Escherichia coli LPS (10, 100 and 1000 ng/kg, consecutively) at 3 week intervals. Intracellular cytokine production was determined by flow cytometry in PBMCs obtained 0, 2, 6 and 24 h after each LPS challenge. After LPS administration, proportions of monocytes producing tumour necrosis factor (TNF) α, interleukin (IL)-1β and IL-8, as well as proportions of circulating lymphocytes producing interferon (IFN) γ, decreased significantly. Within 24 h, proportions had returned to or increased above pre-injection levels. Proportions of lymphocytes producing IL-4 and IL-10 increased significantly after injection of 1000 ng LPS/kg. This study demonstrated that cytokine profiles shift quickly, but temporarily, to favour the anti-inflammatory response immediately after LPS exposure. The long term response to LPS was opposite to the immediate response, as cytokine profiles shifted in the 3 weeks between challenges towards a pro-inflammatory response. Proportions of monocytes producing IL-1β and TNFα determined immediately before the second and/or third LPS injection were higher than proportions determined before the first injection, whereas pre-injection proportions of lymphocytes producing IL-4 decreased with each challenge. These changes may result in a quicker host response to invading pathogens.