The cytokine response of circulating peripheral blood mononuclear cells is changed after intravenous injection of lipopolysaccharide in cattle
The aim of the study was to investigate lipopolysaccharide (LPS)-induced short and long term changes in capacity for intracellular cytokine-production of bovine circulating peripheral blood mononuclear cells (PBMCs). Eight dairy cows each received three intravenous injections of Escherichia coli LPS...
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Published in | The veterinary journal (1997) Vol. 174; no. 1; pp. 170 - 175 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.07.2007
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Subjects | |
Online Access | Get full text |
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Summary: | The aim of the study was to investigate lipopolysaccharide (LPS)-induced short and long term changes in capacity for intracellular cytokine-production of bovine circulating peripheral blood mononuclear cells (PBMCs). Eight dairy cows each received three intravenous injections of
Escherichia coli LPS (10, 100 and 1000
ng/kg, consecutively) at 3
week intervals. Intracellular cytokine production was determined by flow cytometry in PBMCs obtained 0, 2, 6 and 24
h after each LPS challenge. After LPS administration, proportions of monocytes producing tumour necrosis factor (TNF) α, interleukin (IL)-1β and IL-8, as well as proportions of circulating lymphocytes producing interferon (IFN) γ, decreased significantly. Within 24
h, proportions had returned to or increased above pre-injection levels. Proportions of lymphocytes producing IL-4 and IL-10 increased significantly after injection of 1000
ng LPS/kg.
This study demonstrated that cytokine profiles shift quickly, but temporarily, to favour the anti-inflammatory response immediately after LPS exposure. The long term response to LPS was opposite to the immediate response, as cytokine profiles shifted in the 3
weeks between challenges towards a pro-inflammatory response. Proportions of monocytes producing IL-1β and TNFα determined immediately before the second and/or third LPS injection were higher than proportions determined before the first injection, whereas pre-injection proportions of lymphocytes producing IL-4 decreased with each challenge. These changes may result in a quicker host response to invading pathogens. |
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Bibliography: | http://dx.doi.org/10.1016/j.tvjl.2006.06.006 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1090-0233 1532-2971 |
DOI: | 10.1016/j.tvjl.2006.06.006 |