The Expression of Prostaglandin E Receptors EP2 and EP4 and Their Different Regulation by Lipopolysaccharide in C3H/HeN Peritoneal Macrophages

• Published in: The Journal of immunology (1950) Vol. 166; no. 7; pp. 4689 - 4696
• Main Authors: Ikegami, Reiko, Sugimoto, Yukihiko, Segi, Eri, Katsuyama, Masato, Karahashi, Hisae, Amano, Fumio, Maruyama, Takayuki, Yamane, Hana, Tsuchiya, Soken, Ichikawa, Atsushi
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.04.2001
• Subjects: Alprostadil - analogs & derivatives; Alprostadil - pharmacology; Animals; Cyclic AMP - biosynthesis; Dinoprostone - biosynthesis; Dinoprostone - metabolism; Dinoprostone - pharmacology; Down-Regulation - drug effects; Down-Regulation - genetics; Down-Regulation - immunology; Female; Indomethacin - pharmacology; Interleukin-12 - biosynthesis; Lipopolysaccharides - pharmacology; Macrophage Activation - drug effects; Macrophage Activation - genetics; Macrophage Activation - immunology; Macrophages, Peritoneal - enzymology; Macrophages, Peritoneal - immunology; Macrophages, Peritoneal - metabolism; Mice; Mice, Inbred C3H; Prostaglandin-Endoperoxide Synthases - biosynthesis; Prostaglandins E, Synthetic - pharmacology; Receptors, Prostaglandin E - agonists; Receptors, Prostaglandin E - biosynthesis; Receptors, Prostaglandin E - genetics; Receptors, Prostaglandin E, EP4 Subtype; RNA, Messenger - biosynthesis; Tumor Necrosis Factor-alpha - biosynthesis; Up-Regulation - drug effects; Up-Regulation - genetics; Up-Regulation - immunology

The expression and regulation of the PGE receptors, EP(2) and EP(4), both of which are coupled to the stimulation of adenylate cyclase, were examined in peritoneal resident macrophages from C3H/HeN mice. mRNA expression of EP(4) but not EP(2) was found in nonstimulated cells, but the latter was indu...