Synthesis of 9-(beta-D-arabinofuranosyl)guanine using whole cells of Escherichia coli

Synthesis of 9-(beta-D-arabinofuranosyl)guanine (ara-G) from 1-(beta-D-arabinofuranosyl)cytosine (ara-C) and guanine, guanosine or 2'-deoxyguanosine (dG) by glutaraldehyde-treated Escherichia coli BM-11 cells is described. It is shown that the concentration of phosphate ions, molar ratio of sub...

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Bibliographic Details
Published inApplied microbiology and biotechnology Vol. 32; no. 6; p. 658
Main Authors Zinchenko, A I, Barai, V N, Bokut, S B, Kvasyuk, E I, Mikhailopulo, I A
Format Journal Article
LanguageEnglish
Published Germany 01.03.1990
Subjects
Arabinonucleosides - biosynthesis
Arabinonucleosides - isolation & purification
Bacterial Proteins - metabolism
Cytarabine - metabolism
Cytosine Deaminase
Escherichia coli - metabolism
Glutaral
Guanine - metabolism
Guanine Nucleotides - metabolism
Nucleoside Deaminases - metabolism
Purine-Nucleoside Phosphorylase - metabolism
Uridine Phosphorylase - metabolism
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Summary:Synthesis of 9-(beta-D-arabinofuranosyl)guanine (ara-G) from 1-(beta-D-arabinofuranosyl)cytosine (ara-C) and guanine, guanosine or 2'-deoxyguanosine (dG) by glutaraldehyde-treated Escherichia coli BM-11 cells is described. It is shown that the concentration of phosphate ions, molar ratio of substrates and pH of the reaction medium are factors affecting product yield. Under optimum conditions ara-G was produced in the reaction mixture in a yield of 63%-65% based on dG as the best source of guanine base. The yield of isolated ara-G was 48%-53%.
ISSN:0175-7598
DOI:10.1007/BF00164735