GRS, a novel member of the Bcl-2 gene family, is highly expressed in multiple cancer cell lines and in normal leukocytes

• Published in: Oncogene Vol. 14; no. 8; pp. 997 - 1001
• Main Authors: KENNY, J. J, KNOBLOCH, T. J, AUGUSTUS, M, CARTER, K. C, ROSEN, C. A, LANG, J. C
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 27.02.1997; Nature Publishing Group
• Subjects: Apoptosis; Base Sequence; Bcl-2 protein; Biological and medical sciences; Blood Cells - physiology; Cell physiology; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Chromosome 15; Chromosome Mapping; Chromosomes, Human, Pair 15; Deoxyribonucleic acid; DNA; Fibroblast Growth Factor 4; Fibroblast Growth Factors - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression; Gene rearrangement; Genes, bcl-2; Hematopoiesis; Humans; In Situ Hybridization, Fluorescence; Leukocytes; Leukocytes - physiology; Melanoma; Minor Histocompatibility Antigens; Molecular and cellular biology; Molecular Sequence Data; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins c-bcl-2 - genetics; Sequence Alignment; Sequence Homology, Amino Acid; Translocation, Genetic; Tumor cell lines; Tumor Cells, Cultured; Genetic mapping; Human; Nucleotide sequence; Leukocyte; Leukemia; Hematopoietic cell; Homology; Malignant hemopathy; Gene expression; Cell line; Complementary DNA; Chromosome D15; Multigene family; Tumor; Tumor cell; Hybrid gene
• ISSN: 0950-9232; 1476-5594
• DOI: 10.1038/sj.onc.1200898

Our laboratory previously described the independent isolation of the fibroblast growth factor 4 (FGF-4) gene by NIH3T3 transformation assay using DNA from a patient with CML leukemia (Lucas et al., 1994). The FGF-4 gene was truncated by DNA rearrangement with a novel gene named GRS. In this manuscript we describe isolation of GRS cDNA and show by sequence comparison that GRS is a novel member of the Bcl-2 gene family. Northern analysis shows expression of the gene in normal human tissue to be largely restricted to the hematopoietic compartment. Analysis of the pattern of gene expression in cancer cell lines demonstrates GRS is expressed in hematopoietic malignancies and in melanoma. The chromosomal location of GRS has also been determined. The gene is positioned on chromosome 15 within bands q24-25.