Micromonospora lycii sp. nov., a novel endophytic actinomycete isolated from wolfberry root (Lycium chinense Mill)

• Published in: Journal of antibiotics Vol. 69; no. 3; pp. 153 - 158
• Main Authors: Zhao, Junwei, Guo, Lifeng, Liu, Chongxi, Zhang, Yuejing, Guan, Xuejiao, Li, Jiansong, Xu, Shilin, Xiang, Wensheng, Wang, Xiangjing
• Format: Journal Article
• Language: English
• Published: Japan Nature Publishing Group 01.03.2016
• Subjects: Bacterial Typing Techniques; Cell Wall - chemistry; Diaminopimelic Acid - chemistry; DNA, Bacterial - genetics; Fatty Acids - chemistry; Genes, Bacterial; Glycine - chemistry; Lycium - microbiology; Micromonospora - classification; Micromonospora - genetics; Micromonospora - isolation & purification; Peptidoglycan - chemistry; Phospholipids - analysis; Phylogeny; Plant Roots - microbiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Vitamin K 2 - chemistry
• ISSN: 0021-8820; 1881-1469
• DOI: 10.1038/ja.2015.109

A novel actinomycete, designated strain NEAU-gq11(T), was isolated from wolfberry root (Lycium chinense Mill) and characterized using a polyphasic approach. Strain NEAU-gq11(T) was observed to form rough-surfaced spores that borne singly on the substrate hyphae but aerial mycelia were not developed. The organism showed closest 16S rRNA gene sequence similarity to Micromonospora saelicesensis Lupac 09(T) (99.4%), and phylogenetically clustered with M. chokoriensis 2-19/6(T) (99.3%), 'M. zeae' NEAU-gq9(T) (99.3%), M. violae NEAU-zh8(T) (99.1%), 'M. jinlongensis' NEAU-GRX11(T) (99.1%), M. zamorensis CR38(T) (99.0%), M. taraxaci NEAU-P5(T) (98.9%) and M. lupini Lupac 14N(T) (98.6%). Phylogenetic analysis based on the gyrB gene sequences also indicated that the isolate clustered with the above type strains except M. violae NEAU-zh8(T). The cell-wall peptidoglycan consisted of meso-diaminopimelic acid and glycine. The major menaquinones were MK-9(H8), MK-10(H6) and MK-10(H2). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acid was iso-C16:0. Furthermore, some physiological and biochemical properties and low DNA-DNA relatedness values enabled the strain to be differentiated from members of closely related species. Therefore, it is proposed that strain NEAU-gq11(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora lycii sp. nov. is proposed. The type strain is NEAU-gq11(T) (=CGMCC 4.7149(T)=DSM 46703(T)).